Difference between revisions of "Part:BBa K4297056"
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− | plasmid pYLXP' | + | The plasmid pYLXP' contains the expressiom cassette of gene ARO10, ARO9, and PAR4, which can catalyze the reactions of L-phenylalanine to 2-PE. |
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== |
Latest revision as of 02:57, 14 October 2022
PYLXP'-ARO10-ARO8-PAR4
The plasmid pYLXP' contains the expressiom cassette of gene ARO10, ARO9, and PAR4, which can catalyze the reactions of L-phenylalanine to 2-PE.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1293
Illegal NotI site found at 1448 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 5677
Illegal BglII site found at 5884
Illegal BamHI site found at 249
Illegal BamHI site found at 7079
Illegal BamHI site found at 7864
Illegal BamHI site found at 7935
Illegal BamHI site found at 8305
Illegal BamHI site found at 8326
Illegal XhoI site found at 4316
Illegal XhoI site found at 4349
Illegal XhoI site found at 6775
Illegal XhoI site found at 9032 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 4945
Illegal AgeI site found at 950
Illegal AgeI site found at 4585
Illegal AgeI site found at 5535
Illegal AgeI site found at 6410
Illegal AgeI site found at 8031 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1
Illegal BsaI site found at 1284
Illegal BsaI site found at 4588
Illegal BsaI site found at 6413
Illegal BsaI site found at 6467
Illegal BsaI site found at 7069
Illegal BsaI site found at 8034
Illegal BsaI.rc site found at 255
Illegal BsaI.rc site found at 4582
Illegal BsaI.rc site found at 5082
Illegal BsaI.rc site found at 5817
Illegal BsaI.rc site found at 6407
Illegal BsaI.rc site found at 6953
Illegal BsaI.rc site found at 8028
Illegal SapI.rc site found at 7885