Difference between revisions of "Part:BBa K4260031"
| Line 5: | Line 5: | ||
This is a bidirectional promoter region that helps the transcription of two different genes, one in the leader chain of DNA and other in the antisense chain. This type of arrangement is commonly found in operons, in bacteria. This part promotes the transcription of IemR, a trancriptional activator, and together both parts promote the transcription of the coding gene of isoeugenol monooxygenase. | This is a bidirectional promoter region that helps the transcription of two different genes, one in the leader chain of DNA and other in the antisense chain. This type of arrangement is commonly found in operons, in bacteria. This part promotes the transcription of IemR, a trancriptional activator, and together both parts promote the transcription of the coding gene of isoeugenol monooxygenase. | ||
| − | + | [[File:Promotor pseudomonas.jpeg|700px|]] | |
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| + | '''Figure 1.''' Promoter scheme, from Pseudomonas nitroreducens Jin1 | ||
| + | |||
| + | The 2D structure model, obtained with UNAFold, can be observed below, as well as information of its folding thermodynamic values at 37 °C, culture temperature of E. coli. | ||
| + | |||
| + | [[File:Promoter structure.jpeg|700px|]] | ||
| + | |||
| + | '''Figure 2.''' 2D Model of the promoter. Its hairpin formation can be observed. [Generated with UNAFold] | ||
| + | |||
| + | |||
| + | |||
| + | [[File:MAP.jpeg|700px|]] | ||
| + | |||
| + | '''Figure 3.''' Thermodynamic values of the promoter folding. The map shows values of the energy values of the promoter folding at 37°C. | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Latest revision as of 02:41, 14 October 2022
Promoter, for Iem & Iso
This is a bidirectional promoter region that helps the transcription of two different genes, one in the leader chain of DNA and other in the antisense chain. This type of arrangement is commonly found in operons, in bacteria. This part promotes the transcription of IemR, a trancriptional activator, and together both parts promote the transcription of the coding gene of isoeugenol monooxygenase.
Figure 1. Promoter scheme, from Pseudomonas nitroreducens Jin1
The 2D structure model, obtained with UNAFold, can be observed below, as well as information of its folding thermodynamic values at 37 °C, culture temperature of E. coli.
Figure 2. 2D Model of the promoter. Its hairpin formation can be observed. [Generated with UNAFold]
Figure 3. Thermodynamic values of the promoter folding. The map shows values of the energy values of the promoter folding at 37°C.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 164