Difference between revisions of "Part:BBa K4227026:Design"

(→‎Design Notes)
(→‎Design Notes)
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
<p>We design the part as the inhibit module of our oral probiotic. The P<sub>nlmC</sub> was a kind of inducible promoter of the CSP from the <i>S.mutans</i>. We choose it to better control the expression of the muB and sigma28. </p>
+
<p>After an engineering cycle, we failed in verification the muB. And the modeling shows that the express level of the muB is much lower than other proteins. </p>
−
<p>The muB is chosen as a kind of the α-1,3 dextranase to decompose the EPS, and the sigma28 was used to control our kill-switch.</p>
+
<p> In order to have higher level of the expression, we learned the literature and choose the xip-P34,a kind of short peptide which only contains 9 aa. </p>
  
 
===Source===
 
===Source===

Revision as of 01:42, 14 October 2022


PnlmC-xip-P34-sigma28


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

After an engineering cycle, we failed in verification the muB. And the modeling shows that the express level of the muB is much lower than other proteins.

In order to have higher level of the expression, we learned the literature and choose the xip-P34,a kind of short peptide which only contains 9 aa.

Source

Synthesized by the Tsingke Biotechnology Co., Ltd.

References

[1] Tianlei Liu, Shoubin Xue, Wenbo Cai, Xiaojing Liu, Xiaojuan Liu, Rongrong Zheng, Hongyan Luo, Wenhui Qi. ComCED signal loop precisely regulates nlmC expression in Streptococcus mutans. Springer Science and Business Media LLC.2013;1 (64): 31-38.

[2] Lutz, R., and Bujard, H. (1997) Independent and Tight Regulation of Transcriptional Units in Escherichia coli via the LacR/O, the TetR/O and AraC/I-1-I-2 Regulatory Elements. Nucleic Acids Res.25 (6), 1203−1210

[3] Chowdhury Raihan Bikash, Yftah Tal-Gan. Structure Activity Relationship Study of the XIP Quorum Sensing Pheromone in Streptococcus mutans Reveal Inhibitors of the Competence Regulon. American Chemical Society (ACS).2020;10 (15) : 2833-2841. Sequence and Features