Difference between revisions of "Part:BBa K4180002"
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<br>         PCR product </h3>]] | <br>         PCR product </h3>]] | ||
[[File:BBa K4180002-2.png|400px|thumb|left|<h3>After Bacteria transformation, bacteria colonies were picking up directly to do snf1Δ2-306aa - colonies had BBa_K4180002 snf1Δ2-306aa<br>          PCR product </h3>]] | [[File:BBa K4180002-2.png|400px|thumb|left|<h3>After Bacteria transformation, bacteria colonies were picking up directly to do snf1Δ2-306aa - colonies had BBa_K4180002 snf1Δ2-306aa<br>          PCR product </h3>]] | ||
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| + | ==Contribution From NJXDF-CHN 2022== | ||
| + | '''Group''': NJXDF-CHN | ||
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| + | '''Author''': Yang Gu | ||
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| + | '''Summary''': Charactering the effect of the SNF1 gene for lipid synthesis and growth in Y.lipolytica. | ||
| + | ===Characterization from iGEM22-NJXDF-CHN=== | ||
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| + | Sucrose non-fermenting (Snf1) protein kinase, the yeast ortholog of mammalian AMP-activated protein kinase, is the key component of the glucose repression pathway and the energy sensor in yeast. When the ratio of adenosine monophosphate (AMP) to adenosine triphosphate (ATP) is high, it increased catabolic processes to obtain more ATP and decreases anabolic processes to reduce energy consumption. When truncated from the N-terminal amino acid 2 to amino acid 306, the kinase structure of SNF1 can be deleted and the protein inactivated(Liu et al. 2020). It was first registered in 2022. In order to characterize the effect of the IDH2 (BBa_K4297072) gene in lipid synthesis and growth, we constructed a knockout strain po1f ΔylSNF1 using homologous recombination and tested for fermentation in YNB mediums. The experimental results show that the strain po1f ΔylSNF1 can effectively increase the accumulation of biomass and fatty acid content in YNB medium, up to 83.77% and 159.68% at 120h, respectively (Fig.3). These results provide references for future iGEM teams to improve biomass and lipid accumulation in Y.lipolytica. | ||
| + | <html> | ||
| + | <div align="center"> | ||
| + | <figure> | ||
| + | <img src="https://static.igem.wiki/teams/4297/wiki/contribution3new.png" width="100%" style="float:center"> | ||
| + | <figcaption> | ||
| + | <p style="font-size:1rem"> | ||
| + | </p> | ||
| + | </figcaption> | ||
| + | </figure> | ||
| + | </div> | ||
| + | </html> | ||
| + | <div align="center"> | ||
| + | :'''Fig. 3. The growth and lipid synthesis of strain po1f ΔylSNF1 in YNB medium. A. Changes of growth curves in YNB medium. B. Changes of fatty acid content in YNB medium at 120h.''' | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
Latest revision as of 01:39, 14 October 2022
snf1Δ2-306aa N-truncated
snf1Δ2-306aa, N-terminus truncated from amino acid 2 to 306 deleting kinase domain of SNF1, where the phosphorylation takes place on Thr210 to activate the catalytic activity of SNF1
Citation: McCartney, R R, and M C Schmidt. “Regulation of Snf1 kinase. Activation requires phosphorylation of threonine 210 by an upstream kinase as well as a distinct step mediated by the Snf4 subunit.” The Journal of biological chemistry vol. 276,39 (2001): 36460-6. doi:10.1074/jbc.M104418200
Sequence and Features
Contribution From NJXDF-CHN 2022
Group: NJXDF-CHN
Author: Yang Gu
Summary: Charactering the effect of the SNF1 gene for lipid synthesis and growth in Y.lipolytica.
Characterization from iGEM22-NJXDF-CHN
Sucrose non-fermenting (Snf1) protein kinase, the yeast ortholog of mammalian AMP-activated protein kinase, is the key component of the glucose repression pathway and the energy sensor in yeast. When the ratio of adenosine monophosphate (AMP) to adenosine triphosphate (ATP) is high, it increased catabolic processes to obtain more ATP and decreases anabolic processes to reduce energy consumption. When truncated from the N-terminal amino acid 2 to amino acid 306, the kinase structure of SNF1 can be deleted and the protein inactivated(Liu et al. 2020). It was first registered in 2022. In order to characterize the effect of the IDH2 (BBa_K4297072) gene in lipid synthesis and growth, we constructed a knockout strain po1f ΔylSNF1 using homologous recombination and tested for fermentation in YNB mediums. The experimental results show that the strain po1f ΔylSNF1 can effectively increase the accumulation of biomass and fatty acid content in YNB medium, up to 83.77% and 159.68% at 120h, respectively (Fig.3). These results provide references for future iGEM teams to improve biomass and lipid accumulation in Y.lipolytica.
- Fig. 3. The growth and lipid synthesis of strain po1f ΔylSNF1 in YNB medium. A. Changes of growth curves in YNB medium. B. Changes of fatty acid content in YNB medium at 120h.
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