Difference between revisions of "Part:BBa K4438203"
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<partinfo>BBa_K4438203 short</partinfo> | <partinfo>BBa_K4438203 short</partinfo> | ||
| − | Spinach 2.5 is a mutant version of Spinach 2.1(BBa_K1330000), made to increase the fluorescent intensity given out by the aptamer on binding with DFHBI. | + | Spinach 2.5 is a mutant version of Spinach 2.1(<partinfo>BBa_K1330000</partinfo>), made to increase the fluorescent intensity given out by the aptamer on binding with DFHBI. |
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===Usage and Biology=== | ===Usage and Biology=== | ||
| + | The free energy of the thermodynamic ensemble for the sequence below is predicted to be -35.50 kcal/mol. The tetraloop is highlighted in red text and the inversions are underlined. The sequences highlighted in bold are the aptamer sequence and those flanking are the tRNA scaffold sequence, tRNALys3. The mutation and inversions are indicated in the RNAfold structures using blue circles. | ||
| + | [[File:Parts-mod-Figure1_(1).jpg]] | ||
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Latest revision as of 01:17, 14 October 2022
Spinach2.5
Spinach 2.5 is a mutant version of Spinach 2.1(BBa_K1330000), made to increase the fluorescent intensity given out by the aptamer on binding with DFHBI.
Usage and Biology
The free energy of the thermodynamic ensemble for the sequence below is predicted to be -35.50 kcal/mol. The tetraloop is highlighted in red text and the inversions are underlined. The sequences highlighted in bold are the aptamer sequence and those flanking are the tRNA scaffold sequence, tRNALys3. The mutation and inversions are indicated in the RNAfold structures using blue circles.
.jpg)
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]