Difference between revisions of "Part:BBa K210014:Design"

 
(Design Notes)
 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K210014 short</partinfo>
 
<partinfo>BBa_K210014 short</partinfo>
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===Design Notes===
 
===Design Notes===
This parts is useful for MPR products' clonimg
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This plasmid is made from pSB1A2 and primer-dimer.
 
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===Source===
 
===Source===

Latest revision as of 17:52, 15 October 2009

blunt end clonig vector by Stu1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 2070
    Illegal suffix found in sequence at 13
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2070
    Illegal SpeI site found at 14
    Illegal PstI site found at 28
    Illegal NotI site found at 21
    Illegal NotI site found at 2076
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2070
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 2070
    Illegal suffix found in sequence at 14
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 2070
    Illegal XbaI site found at 2085
    Illegal SpeI site found at 14
    Illegal PstI site found at 28
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1109


Design Notes

This plasmid is made from pSB1A2 and primer-dimer.

Source

primer

References