Difference between revisions of "Part:BBa K4390093"
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==Usage and Biology== | ==Usage and Biology== | ||
| − | The soluble version RolA with 6His-tag for Nickel affinity column purification. | + | RolA is part of a class of small fungal proteins called hydrophobins (Puspitasari, et al. 2021). As an 11kDa protein, it has been shown to increase PET hydrolysis, when in the form of a recombinant RolA-PETase. RolA is a highly surface-active substance that assembles spontaneously at hydrophobic-hydrophilic interfaces. By doing so, it is thought that the presence of RolA on PET surfaces makes the hydrophilic PETase able to readily hydrolyse the PET. |
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| + | The soluble version RolA with 6His-tag for Nickel affinity column purification. The wild-type RolA forms insoluble assemblies, so this soluble version of RolA was used to see how this property would affect PETase activity. | ||
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==<span class='h3bb'>Sequence and Features</span>== | ==<span class='h3bb'>Sequence and Features</span>== | ||
<partinfo>BBa_K4390093 SequenceAndFeatures</partinfo> | <partinfo>BBa_K4390093 SequenceAndFeatures</partinfo> | ||
| + | ==Reference== | ||
| + | Puspitasari, N., Tsai, SL. & Lee, CK. Fungal Hydrophobin RolA Enhanced PETase Hydrolysis of Polyethylene Terephthalate. Appl Biochem Biotechnol 193, 1284–1295 (2021). https://doi.org/10.1007/s12010-020-03358-y | ||
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Latest revision as of 00:36, 14 October 2022
His-tagged Soluble RolA
This part is not compatible with BioBrick RFC10 assembly but is compatible with the iGEM Type IIS Part standard which is also accepted by iGEM.
Usage and Biology
RolA is part of a class of small fungal proteins called hydrophobins (Puspitasari, et al. 2021). As an 11kDa protein, it has been shown to increase PET hydrolysis, when in the form of a recombinant RolA-PETase. RolA is a highly surface-active substance that assembles spontaneously at hydrophobic-hydrophilic interfaces. By doing so, it is thought that the presence of RolA on PET surfaces makes the hydrophilic PETase able to readily hydrolyse the PET.
The soluble version RolA with 6His-tag for Nickel affinity column purification. The wild-type RolA forms insoluble assemblies, so this soluble version of RolA was used to see how this property would affect PETase activity.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Reference
Puspitasari, N., Tsai, SL. & Lee, CK. Fungal Hydrophobin RolA Enhanced PETase Hydrolysis of Polyethylene Terephthalate. Appl Biochem Biotechnol 193, 1284–1295 (2021). https://doi.org/10.1007/s12010-020-03358-y