Difference between revisions of "Part:BBa K4387000:Design"

Latest revision as of 20:12, 13 October 2022

Nitric Oxide Sensing Promoter pNorVβ

Assembly Compatibility:

This promoter was tested in a high-copy backbone from Twist Bioscience in the bacterial strain E.coli Nissle 1917.

The sequence of the pNorVβ was obtained from the paper of Xiaoyu J. Chen et al. [1]

[1] Xiaoyu J. Chen, Baojun Wang, Ian P. Thompson, and Wei E. Huang et al. Rational Design and Characterization of Nitric Oxide Biosensors in E. coli Nissle 1917 and Mini SimCells ACS Synthetic Biology 2021 10 (10), 2566-2578 DOI: 10.1021/acssynbio.1c00223

@@ Line 7: / Line 7: @@
 ===Design Notes===
-No codon optimization was necessary. The sequence was taken directly from the reference paper.
+This promoter was tested in a high-copy backbone from Twist Bioscience in the bacterial strain E.coli Nissle 1917.
 ===Source===
-The sequence of the PnorV_beta was obtained from the paper of Xiaoyu J. Chen et al. [1]
+The sequence of the pNorVβ was obtained from the paper of Xiaoyu J. Chen et al. [1]
 ===References===
-[1]Xiaoyu J. Chen, Baojun Wang, Ian P. Thompson, and Wei E. Huang et al. Rational Design and Characterization of Nitric Oxide Biosensors in E. coli Nissle 1917 and Mini SimCells ACS Synthetic Biology 2021 10 (10), 2566-2578 DOI: 10.1021/acssynbio.1c00223
+<ul>
+<li>[1] Xiaoyu J. Chen, Baojun Wang, Ian P. Thompson, and Wei E. Huang et al. Rational Design and Characterization of Nitric Oxide Biosensors in E. coli Nissle 1917 and Mini SimCells ACS Synthetic Biology 2021 10 (10), 2566-2578 <html><a href="https://pubs.acs.org/doi/abs/10.1021/acssynbio.1c00223">DOI: 10.1021/acssynbio.1c00223</a></html></li>
+</ul>