Difference between revisions of "Part:BBa K4167660"

 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K4167660 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4167660 SequenceAndFeatures</partinfo>
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β-galactosidase is encoded by LacZ gene. It is widely found in animals, plants, microorganisms and cultured cells. It can catalyze the hydrolysis of β-galactoside bonds in β-galactoside compounds and release free galactose. It can also cleave lactose to glucose and galactose.
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β-galactosidase is often used as a reporter protein/enzyme for colorimetric assays. For example, β-galactosidase can cleave X-gal to yield galactose and 5-bromo-4-chloro-3-hydroxyindole. The latter is then oxidized to 5-bromo-4-chloro Indigo, a blue color product which is easily measured.
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In addition, β-galactosidase can decompose p-Nitrophenyl-β-D-Galactopyranoside to produce p-Nitrophenol. The product has a characteristic absorption peak at 400 nm. The activity of β-galactosidase can be characterized by the change of absorbance value, which is used to determine the activity of β-galactosidase.
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[[File:K4167660-fig.1.jpg|center]]
  
 
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Revision as of 20:05, 13 October 2022


LacZ generator driven by Plac

This is a β-galactosidase protein generator with strong RBS, driven by Plac promoter. IPTG can bind to the operator site of the promoter to induce β-galactosidase expression. With the different concentration of IPTG, it can express β-galactosidase at different level. With the detection of p-Nitrophenol, the activity of β-galactosidase can be measured.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


β-galactosidase is encoded by LacZ gene. It is widely found in animals, plants, microorganisms and cultured cells. It can catalyze the hydrolysis of β-galactoside bonds in β-galactoside compounds and release free galactose. It can also cleave lactose to glucose and galactose.
β-galactosidase is often used as a reporter protein/enzyme for colorimetric assays. For example, β-galactosidase can cleave X-gal to yield galactose and 5-bromo-4-chloro-3-hydroxyindole. The latter is then oxidized to 5-bromo-4-chloro Indigo, a blue color product which is easily measured.
In addition, β-galactosidase can decompose p-Nitrophenyl-β-D-Galactopyranoside to produce p-Nitrophenol. The product has a characteristic absorption peak at 400 nm. The activity of β-galactosidase can be characterized by the change of absorbance value, which is used to determine the activity of β-galactosidase.

K4167660-fig.1.jpg