Difference between revisions of "Part:BBa K4390070"
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| Untagged Dou-PETase || [[part:BBa_K4390088|K4390088]]
 
| Untagged Dou-PETase || [[part:BBa_K4390088|K4390088]]

Revision as of 19:48, 13 October 2022


This part is not compatible with BioBrick RFC10 assembly but is compatible with the iGEM Type IIS Part standard which is also accepted by iGEM.


Terminator.png

L1U1H08 Synthetic Terminator

  • Synthetic terminator

Secondary Structure

Mfold-K4390070-1.png

Measurement

  • [http://openwetware.org/wiki/Cconboy:Terminator_Characterization/Results How these parts were measured]

Design

A transcription terminator designed for JUMP assembly (Valenzuela-Ortega & French, 2021).

Usage

This terminator was used in following composite parts.

Name Part Number
Untagged Dou-PETase K4390088
N-terminal Car9-tagged Dou-PETase K4390085
Untagged Tri-PETase K4390089
N-terminal L2NC-tagged Tri-PETase K4390115
N-terminal L2NC-linker-tagged Tri-PETase K4390116
N-terminal Car9-tagged Tri-PETase K4390086
C-terminal L2NC-tagged Tri-PETase K4390082
C-terminal L2NC-linker-tagged Tri-PETase K4390083
C-terminal Car9-tagged Tri-PETase K4390081
Untagged FAST-PETase K4390090
N-terminal L2NC-tagged FAST-PETase K4390113
N-terminal L2NC-linker-tagged FAST-PETase K4390114
N-terminal Car9-tagged FAST-PETase K4390084
C-terminal L2NC-tagged FAST-PETase K4390076
C-terminal L2NC-linker-tagged FAST-PETase K4390077
C-terminal Car9-tagged FAST-PETase K4390075
Untagged MHETase K4390091
N-terminal L2NC-tagged MHETase K4390117
N-terminal L2NC-linker-tagged MHETase K4390118
N-terminal Car9-tagged MHETase K4390087

Reference

Marcos Valenzuela-Ortega, Christopher French, Joint universal modular plasmids (JUMP): a flexible vector platform for synthetic biology, Synthetic Biology, Volume 6, Issue 1, 2021, ysab003.