Difference between revisions of "Part:BBa K4212019"

Latest revision as of 18:51, 13 October 2022

Optimal_RBS

Very strong RBS in B. subtilis, increases expression to 25 folds.

Usage and Biology

Ribosome binds to this place and start translation. Optimal RBS can improve the translation efficiency and protein expression level.

References

[1] Nowroozi, F.F., Baidoo, E.E.K., Ermakov, S., Redding-Johanson, A.M., Batth, T.S., Petzold, C.J. & Keasling, J.D. (2014) Metabolic pathway optimization using ribosome binding site variants and combinatorial gene assembly. Applied Microbiology and Biotechnology. 98 (4), 1567–1581. doi:10.1007/s00253-013-5361-4.

[2] McLaughlin, J.R., Murray, C.L. & Rabinowitz, J.C. (1981) Unique features in the ribosome binding site sequence of the gram-positive Staphylococcus aureus beta-lactamase gene. Journal of Biological Chemistry. 256 (21), 11283–11291. doi:10.1016/S0021-9258(19)68589-3.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 5: / Line 5: @@
 Very strong RBS in B. subtilis, increases expression to 25 folds.
-<!-- Add more about the biology of this part here
+<!-- Add more about the biology of this part here-->
 ===Usage and Biology===
+Ribosome binds to this place and start translation. Optimal RBS can improve the translation efficiency and protein expression level.
+===References===
+[1] Nowroozi, F.F., Baidoo, E.E.K., Ermakov, S., Redding-Johanson, A.M., Batth, T.S., Petzold, C.J. & Keasling, J.D. (2014) Metabolic pathway optimization using ribosome binding site variants and combinatorial gene assembly. Applied Microbiology and Biotechnology. 98 (4), 1567–1581. doi:10.1007/s00253-013-5361-4.
+[2] McLaughlin, J.R., Murray, C.L. & Rabinowitz, J.C. (1981) Unique features in the ribosome binding site sequence of the gram-positive Staphylococcus aureus beta-lactamase gene. Journal of Biological Chemistry. 256 (21), 11283–11291. doi:10.1016/S0021-9258(19)68589-3.
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