Difference between revisions of "Part:BBa K176247"

Latest revision as of 16:10, 15 October 2009

High input score automatic selector without lethal device (with reporter): PoPS->mRFP->K176240->PoPS

PoPS->mRFP->luxI-LVA->tetR-LVA + pCon 0.15->luxI-LVA + pCon 0.70->luxR + pLux/Tet->PoPS

Output PoPS = (Cell Density * (pCon 0.15 + Population Average Input PoPS) + Input AHL) / (1 + Input PoPS)

If output is a lethal device, cells with relative high input score in the population will be selected, cells with relative low input score will die.

The first luxI-LVA is used to calculate average score in the population.

mRFP is input score reporter.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 2256
Illegal NheI site found at 2279
Illegal NheI site found at 3105
Illegal NheI site found at 3128
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1387
Illegal BglII site found at 2947
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 573
Illegal AgeI site found at 685
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 4083

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K176247 short</partinfo>
@@ Line 5: / Line 4: @@
 PoPS->mRFP->luxI-LVA->tetR-LVA + pCon 0.15->luxI-LVA + pCon 0.70->luxR + pLux/Tet->PoPS
-Cell Death = (Cell Density * (pCon 0.15 + Population Average Input PoPS) + Input AHL) / (1 + Input PoPS)
+Output PoPS = (Cell Density * (pCon 0.15 + Population Average Input PoPS) + Input AHL) / (1 + Input PoPS)
 If output is a lethal device, cells with relative high input score in the population will be selected, cells with relative low input score will die.