Difference between revisions of "Part:BBa K176249"

 
 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K176249 short</partinfo>
 
<partinfo>BBa_K176249 short</partinfo>
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PoPS->mRFP->cI-LVA+pCI->luxI-LVA->tetR-LVA + pCon 0.15->luxI-LVA + pCon 0.70->luxR + pLux/Tet->PoPS
 
PoPS->mRFP->cI-LVA+pCI->luxI-LVA->tetR-LVA + pCon 0.15->luxI-LVA + pCon 0.70->luxR + pLux/Tet->PoPS
  
Cell Death = (Cell Density * (pCon 0.15 + Population Average (1 / (1 + Input PoPS))) + Input AHL) * Input PoPS
+
Output PoPS = (Cell Density * (pCon 0.15 + Population Average (1 / (1 + Input PoPS))) + Input AHL) * Input PoPS
  
 
If output is a lethal device, cells with relative low input score in the population will be selected, cells with relative high input score will die.
 
If output is a lethal device, cells with relative low input score in the population will be selected, cells with relative high input score will die.

Latest revision as of 16:10, 15 October 2009

Low input score automatic selector w/o lethal device(with reporter): PoPS->mRFP->CI-LVA+pCI->K176240

PoPS->mRFP->cI-LVA+pCI->luxI-LVA->tetR-LVA + pCon 0.15->luxI-LVA + pCon 0.70->luxR + pLux/Tet->PoPS

Output PoPS = (Cell Density * (pCon 0.15 + Population Average (1 / (1 + Input PoPS))) + Input AHL) * Input PoPS

If output is a lethal device, cells with relative low input score in the population will be selected, cells with relative high input score will die.

The first luxI-LVA is used to calculate average score in the population.

mRFP is input score reporter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 3251
    Illegal NheI site found at 3274
    Illegal NheI site found at 4100
    Illegal NheI site found at 4123
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2382
    Illegal BglII site found at 3942
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 573
    Illegal AgeI site found at 685
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 5078