Difference between revisions of "Part:BBa K4438602"
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<partinfo>BBa_K4438602 short</partinfo>
 
<partinfo>BBa_K4438602 short</partinfo>
  
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T6_Target_1 is a single-stranded DNA sequence having 100 nucleotides. Figure 1C) shows the secondary structure and minimum free energy. Its 5’ end has complementary nucleotides for part T6_Trigger_1_phi29 (<partinfo>BBa_K4438601</partinfo>) to bind. The middle region has an ssDNA sense T7 promoter sequence and the 3’ end has an ssDNA sense broccoli light-up aptamer sequence.
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T6_Target_1 is a single-stranded DNA sequence having 100 nucleotides. Figure 1C) shows the secondary structure and minimum free energy. Its 5’ end has complementary nucleotides for part T6_Trigger_1_midtrigger_phi29 (<partinfo>BBa_K4438601</partinfo>) to bind. The middle region has an ssDNA sense T7 promoter sequence and the 3’ end has an ssDNA sense broccoli light-up aptamer sequence.
  
  
 
===Usage and Biology===
 
===Usage and Biology===
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This part along with T6_Trigger_1_phi29 (<partinfo>BBa_K4438601</partinfo>) and Aptamer_T6 (<partinfo>BBa_K4438600</partinfo>) can be used for sensing different concentrations of testosterone.
+
This part along with T6_Trigger_1_midtrigger_phi29 (<partinfo>BBa_K4438601</partinfo>) and Aptamer_T6 (<partinfo>BBa_K4438600</partinfo>) can be used for sensing different concentrations of testosterone.
 
[[File:T--IISER-Tirupati_India--T6_1.png]]
 
[[File:T--IISER-Tirupati_India--T6_1.png]]
  

Revision as of 18:39, 13 October 2022

T6_Target_1

T6_Target_1 is a single-stranded DNA sequence having 100 nucleotides. Figure 1C) shows the secondary structure and minimum free energy. Its 5’ end has complementary nucleotides for part T6_Trigger_1_midtrigger_phi29 (BBa_K4438601) to bind. The middle region has an ssDNA sense T7 promoter sequence and the 3’ end has an ssDNA sense broccoli light-up aptamer sequence.


Usage and Biology

This part along with T6_Trigger_1_midtrigger_phi29 (BBa_K4438601) and Aptamer_T6 (BBa_K4438600) can be used for sensing different concentrations of testosterone. T--IISER-Tirupati India--T6 1.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

Skouridou, V., Jauset-Rubio, M., Ballester, P., Bashammakh, A. S., El-Shahawi, M. S., Alyoubi, A. O., & O’Sullivan, C. K. (2017). Selection and characterization of DNA aptamers against the steroid testosterone. Microchimica Acta, 184(6), 1631-1639.