Difference between revisions of "Part:BBa K4323000:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | We optimize codon for expression in Escherichia coli | + | We optimize the codon for expression in <i>Escherichia coli</i>. The sequence of the Aerolysin was adapted from [https://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1002135 Ioan Iacovache <i>et al</i> (2011)] (1) |
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===Source=== | ===Source=== | ||
| − | Aeromonas hydrophila | + | <i>Aeromonas hydrophila</i> |
===References=== | ===References=== | ||
| + | |||
| + | (1) Iacovache, I.; Degiacomi, M. T.; Pernot, L.; Ho, S.; Schiltz, M.; Dal Peraro, M.; van der Goot, F. G. Dual Chaperone Role of the C-Terminal Propeptide in Folding and Oligomerization of the Pore-Forming Toxin Aerolysin. PLoS Pathogens 2011, 7 (7). | ||
Latest revision as of 18:16, 13 October 2022
Coding sequence for Aerolysin
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 206
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 75
Illegal AgeI site found at 313
Illegal AgeI site found at 1270 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
We optimize the codon for expression in Escherichia coli. The sequence of the Aerolysin was adapted from Ioan Iacovache et al (2011) (1)
Source
Aeromonas hydrophila
References
(1) Iacovache, I.; Degiacomi, M. T.; Pernot, L.; Ho, S.; Schiltz, M.; Dal Peraro, M.; van der Goot, F. G. Dual Chaperone Role of the C-Terminal Propeptide in Folding and Oligomerization of the Pore-Forming Toxin Aerolysin. PLoS Pathogens 2011, 7 (7).