Difference between revisions of "Part:BBa K4307027"

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<partinfo>BBa_K4307027 short</partinfo>
 
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This part is the promoter regulated by PmrB/A two-component system in <i>E.coli</i>. When PmrA (BBa_K4307026) is phosphorylated, it will combine with pmrC and start the gene expression. In Tsinghua 2022 project, it was used in affipmrABC (BBa_K4307028） system.
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This part is the promoter regulated by PmrB/A two-component system in <i>E.coli</i>. When PmrA (BBa_K4307026) is phosphorylated, it will combine with pmrC and start the gene expression. In Tsinghua 2022 project, it was used in affipmrABC (BBa_K4307042） system.
  
  
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<span class='h3bb'>Sequence and Features</span>
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<h2>Sequence and Features</h2>
 
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<partinfo>BBa_K4307000 parameters</partinfo>
 
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<h2>Characterization </h2>
 
<h2>Characterization </h2>
  
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https://static.igem.wiki/teams/4307/wiki/wiki-parts-files/mainpage/bba-k4307027-gel.png<br>
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                </div>
 
                 <b>Figure 1: </b> <b>The construction results of PmrC.</b>
 
                 <b>Figure 1: </b> <b>The construction results of PmrC.</b>
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<!-- Uncomment this to enable Functional Parameter display  
 
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Latest revision as of 16:53, 13 October 2022


PmrC

This part is the promoter regulated by PmrB/A two-component system in E.coli. When PmrA (BBa_K4307026) is phosphorylated, it will combine with pmrC and start the gene expression. In Tsinghua 2022 project, it was used in affipmrABC (BBa_K4307042） system.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 372
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Characterization

The following figure demonstrates our successful construction.


Figure 1: The construction results of PmrC.