Difference between revisions of "Part:BBa K4245203:Design"
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For miRNA 126-3p, we designed two complementary arms, <partinfo>BBa_K4245102</partinfo>, the 3' arm for hsa-miR-126-3p and <partinfo>BBa_K4245109</partinfo>, the 5' arm for hsa-miR-126-3p. For the reporter, we decided on both <partinfo>BBa_K4245130</partinfo>/ <partinfo>BBa_K4245132</partinfo> the FAM and BHQ1 labeled linear probes, and <partinfo>BBa_K4245134</partinfo>/ <partinfo>BBa_K4245135</partinfo>, the DNA fluorescent aptamer split lettuce, due to their frugality and similar wavelength to GFP. | For miRNA 126-3p, we designed two complementary arms, <partinfo>BBa_K4245102</partinfo>, the 3' arm for hsa-miR-126-3p and <partinfo>BBa_K4245109</partinfo>, the 5' arm for hsa-miR-126-3p. For the reporter, we decided on both <partinfo>BBa_K4245130</partinfo>/ <partinfo>BBa_K4245132</partinfo> the FAM and BHQ1 labeled linear probes, and <partinfo>BBa_K4245134</partinfo>/ <partinfo>BBa_K4245135</partinfo>, the DNA fluorescent aptamer split lettuce, due to their frugality and similar wavelength to GFP. | ||
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Nilsson, M., Malmgren, H., Samiotaki, M., Kwiatkowski, M., Chowdhary, B. P., & Landegren, U. (1994). Padlock probes: Circularizing oligonucleotides for localized DNA detection. Science, 265(5181), 2085–2088. https://doi.org/10.1126/science.7522346 | Nilsson, M., Malmgren, H., Samiotaki, M., Kwiatkowski, M., Chowdhary, B. P., & Landegren, U. (1994). Padlock probes: Circularizing oligonucleotides for localized DNA detection. Science, 265(5181), 2085–2088. https://doi.org/10.1126/science.7522346 | ||
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<partinfo>BBa_K4245203 SequenceAndFeatures</partinfo> | <partinfo>BBa_K4245203 SequenceAndFeatures</partinfo> | ||
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Latest revision as of 16:47, 13 October 2022
hsa-miR-126-3p RCA Padlock Probe
A padlock probe, which is often 30-150 nucleotides in length, is a single-stranded DNA sequence designed to recognize a specific target sequence. The “arms” of a padlock probe are the ends of the ssDNA that are complementary to a specific target sequence. The middle sequence (the sequence between the arms) can be specifically designed to perform a function once amplified. (Nilsson et al., 1994)
For miRNA 126-3p, we designed two complementary arms, BBa_K4245102, the 3' arm for hsa-miR-126-3p and BBa_K4245109, the 5' arm for hsa-miR-126-3p. For the reporter, we decided on both BBa_K4245130/ BBa_K4245132 the FAM and BHQ1 labeled linear probes, and BBa_K4245134/ BBa_K4245135, the DNA fluorescent aptamer split lettuce, due to their frugality and similar wavelength to GFP.
References
Nilsson, M., Malmgren, H., Samiotaki, M., Kwiatkowski, M., Chowdhary, B. P., & Landegren, U. (1994). Padlock probes: Circularizing oligonucleotides for localized DNA detection. Science, 265(5181), 2085–2088. https://doi.org/10.1126/science.7522346
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]