Difference between revisions of "Part:BBa K4245210"

 
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__NOTOC__
 
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<partinfo>BBa_K4245210 short</partinfo>
 
<partinfo>BBa_K4245210 short</partinfo>
This part is the T7 regulated miRNA1-broccoli-spacer, designed to test the efficacy of our part, <partinfo>BBa_K4245201</partinfo>, the hsa-miR-1-3p RCT Padlock Probe. We were unsure if the inclusion of a spacer sequence (<partinfo>BBa_K4245160</partinfo>) affected the folding of the broccoli aptamer (<partinfo>BBa_K3380153</partinfo>). The part was designed to imitate the transcript produced when Rolling Circle Transcription (RCT) is applied to hsa-miR-1-3p RCT Padlock Probe.  
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This part is the T7 regulated miRNA1-broccoli-spacer, designed to test the efficacy of the hsa-miR-1-3p RCT Padlock Probe (<partinfo>BBa_K4245201</partinfo>). We were unsure if the inclusion of a spacer sequence (<partinfo>BBa_K4245160</partinfo>) affected the folding of the broccoli aptamer (<partinfo>BBa_K3380153</partinfo>). The part was designed to imitate the transcript produced when Rolling Circle Transcription (RCT) is applied to hsa-miR-1-3p RCT Padlock Probe.  
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 16:40, 13 October 2022


T7 regulated miRNA1-broccoli-spacer
This part is the T7 regulated miRNA1-broccoli-spacer, designed to test the efficacy of the hsa-miR-1-3p RCT Padlock Probe (BBa_K4245201). We were unsure if the inclusion of a spacer sequence (BBa_K4245160) affected the folding of the broccoli aptamer (BBa_K3380153). The part was designed to imitate the transcript produced when Rolling Circle Transcription (RCT) is applied to hsa-miR-1-3p RCT Padlock Probe.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 137
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]