Difference between revisions of "Part:BBa K4307009"

 
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Originated from <i>Lactococcus lactis subsp. Lactis</i>, PnisA is a member of the two-component regulatory system NisK/NisR involved in the regulation of the biosynthesis of lantibiotic nisin. PnisA functions as a promoter which is activated by phosphorylated NisR and enhance downstream gene expression in response to environmental nisin signals. We managed to involve PnisA sequence in plasmids for <i>E.coli</i> strain.<br>
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Functioned together with NisK(BBa_K4307007), NisR(BBa_K4307008), J23100 and EGFP, it forms composite part J23100-nisK-nisR+PnisA-EGFP(BBa_K4307045) to express fluorescence signal induced by nisin.
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<span class='h3bb'>Sequence and Features</span>
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<h2>Sequence and Features</h2>
 
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===Functional Parameters===
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<partinfo>BBa_K4307000 parameters</partinfo>
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<h2>Characterization</h2>
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<p>The following figure demonstrates our successful construction.</p>
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            <a href="https://static.igem.wiki/teams/4307/wiki/wiki-parts-files/mainpage/bba-k4307009-gel.png" class="image">
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                <img alt="" src="https://static.igem.wiki/teams/4307/wiki/wiki-parts-files/mainpage/bba-k4307009-gel.png" width="100%" height=auto class="thumbimage" /></a>                  <div class="thumbcaption">
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                    <a href="https://static.igem.wiki/teams/4307/wiki/wiki-parts-files/mainpage/bba-k4307009-gel.png" class="internal" title="Enlarge"></a>
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                </div>
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                <b>Figure 1: </b> <b>The construction results of PnisA.</b>
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Latest revision as of 16:30, 13 October 2022


PnisA

Originated from Lactococcus lactis subsp. Lactis, PnisA is a member of the two-component regulatory system NisK/NisR involved in the regulation of the biosynthesis of lantibiotic nisin. PnisA functions as a promoter which is activated by phosphorylated NisR and enhance downstream gene expression in response to environmental nisin signals. We managed to involve PnisA sequence in plasmids for E.coli strain.
Functioned together with NisK(BBa_K4307007), NisR(BBa_K4307008), J23100 and EGFP, it forms composite part J23100-nisK-nisR+PnisA-EGFP(BBa_K4307045) to express fluorescence signal induced by nisin.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Characterization

The following figure demonstrates our successful construction.


Figure 1: The construction results of PnisA.