Difference between revisions of "Part:BBa K1404006:Design"
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===References=== | ===References=== | ||
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| + | ==References added by REC-CHENNAI== | ||
| + | Blanco, L. P., Evans, M. L., Smith, D. R., Badtke, M. P., & Chapman, M. R. (2012). Diversity, biogenesis and function of microbial amyloids. Trends in Microbiology, 20(2), 66-73. https://doi.org/10.1016/j.tim.2011.11.005 | ||
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| + | Lin, X., Li, Z., Li, Y., & Lu, Y. (2021). A robust escherichia coli cell-free expression toolbox driven by sigma factors. Biochemical Engineering Journal, 171, 108031. https://doi.org/10.1016/j.bej.2021.108031 | ||
Latest revision as of 15:32, 13 October 2022
p70-CsgA, curli generator
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
If you intend to use this part, be aware that CsgA might be toxic to E. coli cells in large quantities. We were unable to clone csgA in front of the strong promoter J23119 or the weaker promoter J23114
Source
A strong RBS desgined by team Paris Bettencourt 2009 and the coding region for the csgA gene as found in the genome of the E. coli strain MC4100 were synthesized by Genecust. An illegal restriction site was removed by introducing a silent mutation. This direct synthesis was then cloned downstream of the K342000 promoter by standard assembly.
References
References added by REC-CHENNAI
Blanco, L. P., Evans, M. L., Smith, D. R., Badtke, M. P., & Chapman, M. R. (2012). Diversity, biogenesis and function of microbial amyloids. Trends in Microbiology, 20(2), 66-73. https://doi.org/10.1016/j.tim.2011.11.005
Lin, X., Li, Z., Li, Y., & Lu, Y. (2021). A robust escherichia coli cell-free expression toolbox driven by sigma factors. Biochemical Engineering Journal, 171, 108031. https://doi.org/10.1016/j.bej.2021.108031