Difference between revisions of "Part:BBa K259006"

Revision as of 14:20, 13 October 2022

GFP-Terminator

This is a composite part made from the reporter gene GFP and a added on to a double terminator. It is ideal for adding on at the end of your constructs. See details below.

GFP Structure

The Structure of GFP. Image courtesy of http://www.wwpdb.org/ PDB

Terminator Structure

The structure/sequence of the double terminator. Image from the original part page

Part Description

This is a composite part made from Green Fluorescent Protein (GFP) and a double terminator.

Green Fluorescent protein (originally from part BBa_E0040)will glow green when excited by the correct wavelengths and thus can be used as a reporter gene. This part is directly fused to a terminator (originally from part BBa_B0014) so it is ideal to be fused at the end of your construct.

Table of Statistics

References

<biblio>

1. Valdivia pmid=8707053

</biblio>

1)Chalfie, M., & Kain, S. R. (2005). Green fluorescent protein: Properties, applications and protocols. John Wiley & Sons.

2)Green fluorescent protein as a signal for protein-protein interactions - https://onlinelibrary.wiley.com/doi/abs/10.1002/pro.5560061107

3)Kukar, T., Eckenrode, S., Gu, Y., Lian, W., Megginson, M., She, J., & Wu, D. (2002). Protein microarrays to detect protein-protein interactions using red and green fluorescent proteins. Analytical Biochemistry, 306(1), 50-54. https://doi.org/10.1006/abio.2002.5614

4)Park, S., & Raines, R. T. (2008). Green fluorescent protein as a signal for protein-protein interactions. Protein Science, 6(11), 2344-2349. https://doi.org/10.1002/pro.5560061107

Sequence and Features