Difference between revisions of "Part:BBa K4497014"

 
 
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<partinfo>BBa_K4497014 short</partinfo>
 
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This part encodes the sequence for a mCherry-nanobody based receptor for secretion/ surface expression. The receptor contains a Flag- and HA-tag for immunofluorescence labeling.
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==Design & Cloning==
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===Design===
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This part is made of the following components:
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*mCherry nanobody receptor ([[Part:BBa_K4497014]])
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** IL-11 Signaling Sequence ([[Part:BBa_K4497003]]): Signaling Sequence for Cell surface expression
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** Flag-tag ([[Part:BBa_K4497012]]): Flag epitope tag that enables surface expression detection using immunofluorescence labeling
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** HA-tag ([[Part:BBa_K1150016]]): HA epitope tag that enables surface expression detection using immunofluorescence labeling
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** Anti-mCherry nanobody ([[Part:BBa_K4497002]]): a nanobody capable of binding mCherry
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===Cloning===
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The part is encoded on the plasmid  pcDNA3.1-SP-Flag HA-Tag-mCherrynb-gp130 which was kindly provided to us by Prof. Scheller [1]
  
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K4497014 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4497014 SequenceAndFeatures</partinfo>
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===References===
  
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[1] Mossner, S., Phan, H. T., Triller, S., Moll, J. M., Conrad, U., & Scheller, J. (2020). Multimerization strategies for efficient production and purification of highly active synthetic cytokine receptor ligands. PLOS ONE, 15(4), e0230804.
  
 
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Latest revision as of 14:04, 13 October 2022


mCherry Nanobody Receptor

This part encodes the sequence for a mCherry-nanobody based receptor for secretion/ surface expression. The receptor contains a Flag- and HA-tag for immunofluorescence labeling.

Design & Cloning

Design

This part is made of the following components:

  • mCherry nanobody receptor (Part:BBa_K4497014)
    • IL-11 Signaling Sequence (Part:BBa_K4497003): Signaling Sequence for Cell surface expression
    • Flag-tag (Part:BBa_K4497012): Flag epitope tag that enables surface expression detection using immunofluorescence labeling
    • HA-tag (Part:BBa_K1150016): HA epitope tag that enables surface expression detection using immunofluorescence labeling
    • Anti-mCherry nanobody (Part:BBa_K4497002): a nanobody capable of binding mCherry

Cloning

The part is encoded on the plasmid pcDNA3.1-SP-Flag HA-Tag-mCherrynb-gp130 which was kindly provided to us by Prof. Scheller [1]

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 373
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 373
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 94
    Illegal BamHI site found at 127
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 373
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 373
  • 1000
    COMPATIBLE WITH RFC[1000]

References

[1] Mossner, S., Phan, H. T., Triller, S., Moll, J. M., Conrad, U., & Scheller, J. (2020). Multimerization strategies for efficient production and purification of highly active synthetic cytokine receptor ligands. PLOS ONE, 15(4), e0230804.