Difference between revisions of "Part:BBa K4497008"

 
 
(2 intermediate revisions by the same user not shown)
Line 3: Line 3:
 
<partinfo>BBa_K4497008 short</partinfo>
 
<partinfo>BBa_K4497008 short</partinfo>
  
−
v
+
This part encodes the sequence for a GFP-nanobody based receptor for secretion/ surface expression. The receptor contains a myc-tag for immunofluorescence labeling.
 +
 
 +
==Design & Cloning==
 +
===Design===
 +
This part is made of the following components:
 +
 
 +
* IL-11 Signaling Sequence ([[Part:BBa_K4497003]]): Signaling Sequence for Cell surface expression
 +
* Myc-tag ([[Part:BBa_K112502]]): Myc epitope tag that enables surface expression detection using immunofluorescence labeling
 +
* Anti-GFP nanobody ([[Part:BBa_K4497002]]): a nanobody capable of binding GFP
 +
 
 +
===Cloning===
 +
The part is encoded on the plasmid  pcDNA3.1-myc-GFPnb-gp130 which was kindly provided to us by Prof. Scheller [1]
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
Line 11: Line 22:
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K4497008 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4497008 SequenceAndFeatures</partinfo>
 +
===References===
  
 +
[1] Mossner, S., Phan, H. T., Triller, S., Moll, J. M., Conrad, U., & Scheller, J. (2020). Multimerization strategies for efficient production and purification of highly active synthetic cytokine receptor ligands. PLOS ONE, 15(4), e0230804.
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  

Latest revision as of 14:03, 13 October 2022


GFP Nanobody Receptor

This part encodes the sequence for a GFP-nanobody based receptor for secretion/ surface expression. The receptor contains a myc-tag for immunofluorescence labeling.

Design & Cloning

Design

This part is made of the following components:

  • IL-11 Signaling Sequence (Part:BBa_K4497003): Signaling Sequence for Cell surface expression
  • Myc-tag (Part:BBa_K112502): Myc epitope tag that enables surface expression detection using immunofluorescence labeling
  • Anti-GFP nanobody (Part:BBa_K4497002): a nanobody capable of binding GFP

Cloning

The part is encoded on the plasmid pcDNA3.1-myc-GFPnb-gp130 which was kindly provided to us by Prof. Scheller [1]

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 349
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 349
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 103
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 349
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 349
  • 1000
    COMPATIBLE WITH RFC[1000]

References

[1] Mossner, S., Phan, H. T., Triller, S., Moll, J. M., Conrad, U., & Scheller, J. (2020). Multimerization strategies for efficient production and purification of highly active synthetic cytokine receptor ligands. PLOS ONE, 15(4), e0230804.