Difference between revisions of "Part:BBa K4497008"
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K4497008 SequenceAndFeatures</partinfo> | <partinfo>BBa_K4497008 SequenceAndFeatures</partinfo> | ||
| + | ===References=== | ||
| + | [1] Mossner, S., Phan, H. T., Triller, S., Moll, J. M., Conrad, U., & Scheller, J. (2020). Multimerization strategies for efficient production and purification of highly active synthetic cytokine receptor ligands. PLOS ONE, 15(4), e0230804. | ||
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Revision as of 13:53, 13 October 2022
GFP Nanobody Receptor
This part encodes the seqeunce for a GFP-nanobody based receptor for secretion/ surface expression. The receptor contains a myc-tag for immunofluorescence labeling.
Design & Cloning
Design
This part is made of the following components:
- IL-11 Signaling Sequence (Part:BBa_K4497003): Signaling Sequence for Cell surface expression
- Myc-tag (Part:BBa_K112502): Myc epitope tag that enables surface expression detection using immunofluorescence labeling
- Anti-GFP nanobody (Part:BBa_K4497002): a nanobody capable of binding GFP
Cloning
The part is encoded on the plasmid pcDNA3.1-myc-GFPnb-gp130 which was kindly provided to us by Prof. Scheller [1]
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 349
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 349
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 103
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 349
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 349
- 1000COMPATIBLE WITH RFC[1000]
References
[1] Mossner, S., Phan, H. T., Triller, S., Moll, J. M., Conrad, U., & Scheller, J. (2020). Multimerization strategies for efficient production and purification of highly active synthetic cytokine receptor ligands. PLOS ONE, 15(4), e0230804.