Difference between revisions of "Part:BBa K4165168"
 
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<partinfo>BBa_K4165168 short</partinfo>
 
<partinfo>BBa_K4165168 short</partinfo>
  
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This composite part consists of T7 promoter (BBa_K3633015), lac operator (BBa_K4165062), AB-RBS (BBa_K4165095), 6x His-tag (BBa_K4165020), amyloid beta (BBa_K4165005), and T7 terminator (BBa_K731721), The His tag was attached to the Amyloid beta coding sequence to serve in the purification using NI-NTA column.
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This composite part consists of T7 promoter (BBa_K3633015), lac operator (BBa_K4165062), RBS 6 (BBa_K4165095), 6x His-tag (BBa_K4165020), amyloid beta (BBa_K4165005), and T7 terminator (BBa_K731721), The His tag was attached to the Amyloid beta coding sequence to serve in the purification using NI-NTA column.
  
 
===Usage and Biology===
 
===Usage and Biology===
 
Amyloid β Protein Fragment 1-42 results from the amyloidogenic degradation of Amyloid beta precursor and forms toxic plaques inside the brain leading to the development of Alzheimer's Disease, this protein which we target to degrade in our project.
 
Amyloid β Protein Fragment 1-42 results from the amyloidogenic degradation of Amyloid beta precursor and forms toxic plaques inside the brain leading to the development of Alzheimer's Disease, this protein which we target to degrade in our project.
  
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===Source===
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===<span class='h3bb'>Sequence and Features</span>===
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Synthesized
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<partinfo>BBa_K4165168 SequenceAndFeatures</partinfo>
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===Dry Lab===
 
===Dry Lab===
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                   Figure 1. this figure shows the results from the transcription and translation code showing the  
 
                   Figure 1. this figure shows the results from the transcription and translation code showing the  
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                      variation of mRNA and protein concentrations with time compared with the wet lab results.
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                                    variation of mRNA and protein concentrations with time.
  
 
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<span class='h3bb'>Sequence and Features</span>
 
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<partinfo>BBa_K4165168 SequenceAndFeatures</partinfo>
 
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<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  
 
===Functional Parameters===
 
===Functional Parameters===
 
<partinfo>BBa_K4165168 parameters</partinfo>
 
<partinfo>BBa_K4165168 parameters</partinfo>
 
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Latest revision as of 13:44, 13 October 2022


T7 promoter- RBS-6x His tag- amyloid beta- T7 terminator

This composite part consists of T7 promoter (BBa_K3633015), lac operator (BBa_K4165062), RBS 6 (BBa_K4165095), 6x His-tag (BBa_K4165020), amyloid beta (BBa_K4165005), and T7 terminator (BBa_K731721), The His tag was attached to the Amyloid beta coding sequence to serve in the purification using NI-NTA column.

Usage and Biology

Amyloid β Protein Fragment 1-42 results from the amyloidogenic degradation of Amyloid beta precursor and forms toxic plaques inside the brain leading to the development of Alzheimer's Disease, this protein which we target to degrade in our project.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Dry Lab

Mathematical modeling

Transcription rate and translation rate under T7 promotor

the mathematical modeling was based on our code for the calculation of transcription and translation (you can find it in the code section) beside with the estimated results from the wet lab. 

                  Figure 1. this figure shows the results from the transcription and translation code showing the 
                                   variation of mRNA and protein concentrations with time.