Difference between revisions of "Part:BBa K4165149"
 
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<partinfo>BBa_K4165149 short</partinfo>
 
<partinfo>BBa_K4165149 short</partinfo>
  
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This composite part consists of T7 promoter (BBa_K3633015), lac operator (BBa_K4165062), pGS-21a RBS (BBa_K4165016), 6x His-tag (BBa_K4165020), HtrA1 (BBa_K4165004), and T7 terminator (BBa_K731721),The His tag was attached to the HTRA1 coding sequence to serve in the purification using NI-NTA column.
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This composite part consists of T7 promoter (BBa_K3633015), lac operator (BBa_K4165062), pET22b RBS (BBa_K2924053), 6x His-tag (BBa_K4165020), HtrA1 (BBa_K4165004), and T7 terminator (BBa_K731721),The His tag was attached to the HTRA1 coding sequence to serve in the purification using NI-NTA column.
  
 
===Usage and Biology===
 
===Usage and Biology===
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the expression of serine protease HtrA1 which is an ATP-independent protease that can work extra- and intracellularly, It has been used in this project to target and degrade both tau and Aβ proteins which are both considered main causes of Alzheimer’s Disease pathogenesis.
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the expression of serine protease HtrA1 which is an ATP-independent protease that can work extra- and intracellularly, It has been used in this project to target and degrade both tau and Aβ proteins which are both considered main causes of Alzheimer’s Disease pathogenesis.
  
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===Source===
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===<span class='h3bb'>Sequence and Features</span>===
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Synthesized
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<partinfo>BBa_K4165149 SequenceAndFeatures</partinfo>
  
 
===Dry Lab===
 
===Dry Lab===
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                     Figure 1. this figure shows the results from the transcription and translation code showing the  
 
                     Figure 1. this figure shows the results from the transcription and translation code showing the  
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                      variation of mRNA and protein concentrations with time compared with the wet lab results.
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                                      variation of mRNA and protein concentrations with time .
 
   
 
   
 
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<span class='h3bb'>Sequence and Features</span>
 
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<partinfo>BBa_K4165149 SequenceAndFeatures</partinfo>
 
  
  

Latest revision as of 13:44, 13 October 2022


T7 promoter-6x His tag-HtrA1-T7 terminator

This composite part consists of T7 promoter (BBa_K3633015), lac operator (BBa_K4165062), pET22b RBS (BBa_K2924053), 6x His-tag (BBa_K4165020), HtrA1 (BBa_K4165004), and T7 terminator (BBa_K731721),The His tag was attached to the HTRA1 coding sequence to serve in the purification using NI-NTA column.

Usage and Biology

the expression of serine protease HtrA1 which is an ATP-independent protease that can work extra- and intracellularly, It has been used in this project to target and degrade both tau and Aβ proteins which are both considered main causes of Alzheimer’s Disease pathogenesis.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 181
  • 1000
    COMPATIBLE WITH RFC[1000]

Dry Lab

Mathematical modeling

Transcription rate and translation rate under T7 promotor

the mathematical modeling was based on our code for the calculation of transcription and translation (you can find it in the code section) beside with the estimated results from the wet lab. 

                   Figure 1. this figure shows the results from the transcription and translation code showing the 
                                     variation of mRNA and protein concentrations with time .