Difference between revisions of "Part:BBa K4307027"

Revision as of 12:21, 13 October 2022

PmrC

This part is the promoter regulated by PmrB/A two-component system in E.coli. When PmrA (BBa_K4307026) is phosphorylated, it will combine with pmrC and start the gene expression. In Tsinghua 2022 project, it was used in affipmrABC (BBa_K4307028） system.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 372
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Characterization

The following figure demonstrates our successful construction.

Figure 1: The construction results of PmrC.

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+    <div class="thumb tnone">
+        <div class="thumbinner" style="width:50%;">
+            <a href="https://static.igem.wiki/teams/4307/wiki/wiki-parts-files/mainpage/bba-k4307027-gel.png" class="image">
+                <img alt="" src="https://static.igem.wiki/teams/4307/wiki/wiki-parts-files/mainpage/bba-k4307027-gel.png" width="100%" height=auto class="thumbimage" /></a>                  <div class="thumbcaption">
+                <div class="magnify">
+                    <a href="https://static.igem.wiki/teams/4307/wiki/wiki-parts-files/mainpage/bba-k4307027-gel.png" class="internal" title="Enlarge"></a>
+                </div>
                  <b>Figure 1: </b> <b>The construction results of PmrC.</b>
+            </div>
+        </div>
+    </div>
+</div>
+</html>
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