Difference between revisions of "Part:BBa K4497002"

Latest revision as of 11:56, 13 October 2022

Anti-GFP nanobody

Anti-GFP nanobody is a nanobody directed against GFP [1].

When using this part consider:

Add Signaling Sequence, there is no Start Codon, there is no Stop Codon

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 241
12
INCOMPATIBLE WITH RFC[12]
Illegal PstI site found at 241
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 241
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 241
1000
COMPATIBLE WITH RFC[1000]

References

[1]: Zhang, Z., Wang, Y., Ding, Y., & Hattori, M. (2020). Structure-based engineering of anti-GFP nanobody tandems as ultra-high-affinity reagents for purification. Scientific Reports, 10(1), 1–10. https://doi.org/10.1038/s41598-020-62606-7

@@ Line 2: / Line 2: @@
 __NOTOC__
 <partinfo>BBa_K4497002 short</partinfo>
-Add Signaling Sequence
-No Start Codon
+Anti-GFP nanobody is a nanobody directed against GFP [1].
-No Stop Codon
+When using this part consider:
+Add Signaling Sequence, there is no Start Codon, there is no Stop Codon
 <!-- Add more about the biology of this part here
@@ Line 14: / Line 18: @@
+===References===
+[1]: Zhang, Z., Wang, Y., Ding, Y., & Hattori, M. (2020). Structure-based engineering of anti-GFP nanobody tandems as ultra-high-affinity reagents for purification. Scientific Reports, 10(1), 1–10. https://doi.org/10.1038/s41598-020-62606-7
 <!-- Uncomment this to enable Functional Parameter display
 ===Functional Parameters===
 <partinfo>BBa_K4497002 parameters</partinfo>
 <!-- -->