Difference between revisions of "Part:BBa K4165096"
 
(9 intermediate revisions by 3 users not shown)
Line 3: Line 3:
 
<partinfo>BBa_K4165096 short</partinfo>
 
<partinfo>BBa_K4165096 short</partinfo>
  
−
part encodes for a synthetic peptide that binds to the PDZ domain of HTRA1 protein
+
Part encodes a synthetic peptide that binds to the PDZ domain of HTRA1 protein
  
 
===Usage and Biology===
 
===Usage and Biology===
  
−
Synthetic peptide acquired by Phage display, Binds specifically to PDZ domain of HTRA1 and HTRA3 Proteins with ΔG of –8.5 kJ.mol<sup>-1</sup> and IC<sub>50</sub> of 1.3 ± 0.1
+
Synthetic peptide acquired by Phage display, Binds specifically to PDZ domain of HTRA1 and HTRA3 Proteins with ΔG of –8.5 Kcal/mol and IC<sub>50</sub> of 1.3 ± 0.1
−
 
+
  
 
<!-- -->
 
<!-- -->
Line 15: Line 14:
  
  
−
===Functional Parameters===
+
===Dry Lab Characterization===
 +
<p style=" font-weight: bold; font-size:14px;"> Modeling </p>
 +
 
 
Got score of 5 out of 6 in our Quality assessment code after modeling by AlphaFold2.0
 
Got score of 5 out of 6 in our Quality assessment code after modeling by AlphaFold2.0
 +
  
 
<html>
 
<html>
Line 47: Line 49:
 
</body>
 
</body>
  
−
<p style=" font-weight: bold; font-size:14px;"> Modeling </p>
+
 
 +
 
 +
 
 
<html>
 
<html>
−
<p><img src="https://static.igem.wiki/teams/4165/wiki/parts-registry/htra1-bp/h1b.jpg" style="margin-left:200px;" alt="" width="500" /></p>
+
<p><img src="https://static.igem.wiki/teams/4165/wiki/parts-registry/htra1-bp/h1b.jpg" style="margin-left:220px;" alt="" width="500" /></p>
 
</html>
 
</html>
  
−
                   Figure 1.: 3D Model of H1B peptide Modelled by AlphaFold2.0 displayed in Pyomol
+
                   Figure 1.: 3D Model of H1B peptide Modelled by AlphaFold2.0 displayed in Pymol
  
 +
===References===
 +
1.Romero-Molina, S., Ruiz-Blanco, Y. B., Mieres-Perez, J., Harms, M., Münch, J., Ehrmann, M., & Sanchez-Garcia, E. (2022). PPI-Affinity: A Web Tool for the Prediction and Optimization of Protein–Peptide and Protein–Protein Binding Affinity. Journal of Proteome Research.
 +
2.Runyon, S. T., Zhang, Y., Appleton, B. A., Sazinsky, S. L., Wu, P., Pan, B., ... & Sidhu, S. S. (2007). Structural and functional analysis of the PDZ domains of human HtrA1 and HtrA3. Protein Science, 16(11), 2454-2471.
  
 
<partinfo>BBa_K4165096 parameters</partinfo>
 
<partinfo>BBa_K4165096 parameters</partinfo>
 
<!-- -->
 
<!-- -->

Latest revision as of 11:08, 13 October 2022


HTRA Binding Peptide 2

Part encodes a synthetic peptide that binds to the PDZ domain of HTRA1 protein

Usage and Biology

Synthetic peptide acquired by Phage display, Binds specifically to PDZ domain of HTRA1 and HTRA3 Proteins with ΔG of –8.5 Kcal/mol and IC50 of 1.3 ± 0.1

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Dry Lab Characterization

Modeling

Got score of 5 out of 6 in our Quality assessment code after modeling by AlphaFold2.0


cbeta_deviations molprobity ramachandran_favored ramachandran_outliers Qmean_4 Qmean_6
0 4.26 40 60 -6.45183 -5.51397 

                 Figure 1.: 3D Model of H1B peptide Modelled by AlphaFold2.0 displayed in Pymol

References

1.Romero-Molina, S., Ruiz-Blanco, Y. B., Mieres-Perez, J., Harms, M., Münch, J., Ehrmann, M., & Sanchez-Garcia, E. (2022). PPI-Affinity: A Web Tool for the Prediction and Optimization of Protein–Peptide and Protein–Protein Binding Affinity. Journal of Proteome Research. 2.Runyon, S. T., Zhang, Y., Appleton, B. A., Sazinsky, S. L., Wu, P., Pan, B., ... & Sidhu, S. S. (2007). Structural and functional analysis of the PDZ domains of human HtrA1 and HtrA3. Protein Science, 16(11), 2454-2471.