Difference between revisions of "Part:BBa K4165119"

Latest revision as of 09:21, 13 October 2022

HTRA Binding Peptide 25

Part encodes for a synthetic peptide that binds to the PDZ domain of HTRA1 protein.

Usage and Biology

Synthetic peptide acquired by Phage display, Binds specifically to the water-sites of PDZ domain of HTRA1 Protein with IC₅₀ of 0.16 ± 0.01 and ΔG of 8.0 kcal/mol.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Dry Lab Characterization

Modeling

Got score of 4 out of 6 in our Quality assessment code after modeling by AlphaFold2.0

cbeta_deviations	clashscore	molprobity	ramachandran_favored	ramachandran_outliers	Qmean_4	Qmean_6
0	0	2.64	0	0	-6.45183	-5.513969

                    Figure 1.: 3D Model of H1Y peptide Modelled by AlphaFold2.0 displayed in Pymol.

References

Beuming, T., Farid, R., & Sherman, W. (2009). High‐energy water sites determine peptide binding affinity and specificity of PDZ domains. Protein Science, 18(8), 1609-1619.

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 ===Usage and Biology===
-Synthetic peptide acquired by Phage display, Binds specifically to the water-sites of PDZ domain of HTRA1 Protein with IC<sub>50</sub> of 0.16 ± 0.01 and ΔG of 8.0 kJ.mol<sup>-1</sup>.
+Synthetic peptide acquired by Phage display, Binds specifically to the water-sites of PDZ domain of HTRA1 Protein with IC<sub>50</sub> of 0.16 ± 0.01 and ΔG of 8.0 kcal/mol.
 <!-- -->
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 Got score of 4 out of 6 in our Quality assessment code after modeling by AlphaFold2.0
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-<p><img src="https://static.igem.wiki/teams/4165/wiki/parts-registry/htra1-bp/h1y.jpg" style="margin-left:200px;" alt="" width="500" /></p>
+<p><img src="https://static.igem.wiki/teams/4165/wiki/parts-registry/htra1-bp/h1y.jpg" style="margin-left:250px;" alt="" width="500" /></p>
 </html>