Difference between revisions of "Part:BBa K4195069"

Latest revision as of 09:05, 13 October 2022

pirA_i

This sequence is a conserved region of toxin gene pirA(1). It’s used as the detection target of RENDR system.

Biology

Ribozyme ENabled Detection of RNA (RENDR)
RENDR is a high-performing, plug-and-play RNA-sensing platform(1). RENDR utilizes a split variant of the Tetrahymena thermophila ribozyme by synthetically splitting it into two non-functional fragments (Fig. 1). Two fragments are each appended with designed RNA guide sequences, which can interact with the RNA input of interest. The split ribozyme is then inserted within a desired gene output. When bound with the RNA input, two transcribed split ribozyme fragments are triggered to self-splice and thus the intact transcript of the protein output will form.

Fig. 1 Schematic illustration of RENDR.

Usage and Design

This part is used as the target of the RENDR detection system. For toxin pirA, we designed BBa_K4195141, BBa_K4195142, BBa_K4195145, BBa_K4195146, BBa_K4195156, BBa_K4195157, BBa_K4195160, BBa_K4195161, BBa_K4195168, BBa_K4195169, BBa_K4195172, BBa_K4195173. Other related parts are as following: BBa_K4195151, BBa_K4195183, BBa_K4195184, BBa_K4195185, BBa_K4195186.

Reference

1. J. M. S. Lazarte et al., Passive Immunization with Recombinant Antibody VLRB-PirA(vp)/PirB(vp)-Enriched Feeds against Vibrio parahaemolyticus Infection in Litopenaeus vannamei Shrimp. Vaccines (Basel) 9, (2021). Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 __NOTOC__
 <partinfo>BBa_K4195069 short</partinfo>
+This sequence is a conserved region of toxin gene <i>pirA</i>(<i>1</i>). It’s used as the detection target of RENDR system.
 ===Biology===
-This sequence is a conserved region of toxin gene <i>pirA</i>(<i>1</i>). It’s used as the detection target of RENDR system.
-<br>
 <b>Ribozyme ENabled Detection of RNA (RENDR)</b>
 <br>
@@ Line 12: / Line 13: @@
 <br>
 <b>Fig. 1 Schematic illustration of RENDR.</b>
-<!-- Add more about the biology of this part here
 ===Usage and Design===
 <br>
-This part is used as the target of the RENDR detection system. For toxin <i>pirA</i>, we designed <partinfo>BBa_K4195141</partinfo>, <partinfo>BBa_K4195142</partinfo>, <partinfo>BBa_K4195145</partinfo>, <partinfo>BBa_K4195146</partinfo>, <partinfo>BBa_K4195156</partinfo>, <partinfo>BBa_K4195157</partinfo>, <partinfo>BBa_K4195160</partinfo>, <partinfo>BBa_K4195161</partinfo>, <partinfo>BBa_K4195168</partinfo>, <partinfo>BBa_K4195169</partinfo>, <partinfo>BBa_K4195172</partinfo>, <partinfo>BBa_K4195173</partinfo>. Other related parts are as followings: <partinfo>BBa_K4195151</partinfo>, <partinfo>BBa_K4195183</partinfo>, <partinfo>BBa_K4195184</partinfo>, <partinfo>BBa_K4195185</partinfo>, <partinfo>BBa_K4195186</partinfo>.
+This part is used as the target of the RENDR detection system. For toxin <i>pirA</i>, we designed <partinfo>BBa_K4195141</partinfo>, <partinfo>BBa_K4195142</partinfo>, <partinfo>BBa_K4195145</partinfo>, <partinfo>BBa_K4195146</partinfo>, <partinfo>BBa_K4195156</partinfo>, <partinfo>BBa_K4195157</partinfo>, <partinfo>BBa_K4195160</partinfo>, <partinfo>BBa_K4195161</partinfo>, <partinfo>BBa_K4195168</partinfo>, <partinfo>BBa_K4195169</partinfo>, <partinfo>BBa_K4195172</partinfo>, <partinfo>BBa_K4195173</partinfo>. Other related parts are as following: <partinfo>BBa_K4195151</partinfo>, <partinfo>BBa_K4195183</partinfo>, <partinfo>BBa_K4195184</partinfo>, <partinfo>BBa_K4195185</partinfo>, <partinfo>BBa_K4195186</partinfo>.
 ===Reference===
 <br/>
-[1] Lazarte, J., Kim, Y. R., Lee, J. S., Chun, J. H., Kim, S. W., Jung, J. W., Kim, J., Kayansamruaj, P., Thompson, K. D., Kim, H., & Jung, T. S. (2021). Passive Immunization with Recombinant Antibody VLRB-PirA<sup>vp</sup>/PirB<sup>vp</sup>-Enriched Feeds against ''Vibrio parahaemolyticus'' Infection in ''Litopenaeus'' ''vannamei'' Shrimp. ''Vaccines'', 9(1), 55. https://doi.org/10.3390/vaccines9010055
+. J. M. S. Lazarte <i>et al.</i>, Passive Immunization with Recombinant Antibody VLRB-PirA(vp)/PirB(vp)-Enriched Feeds against <i>Vibrio parahaemolyticus</i> Infection in <i>Litopenaeus vannamei</i> Shrimp. <i>Vaccines (Basel)</i> <b>9</b>,  (2021).
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 <span class='h3bb'>Sequence and Features</span>