Difference between revisions of "Part:BBa K4165208"

 
 
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Fusion protein consists of Tau binding peptide (M4W39) for tau targeting and Coh2 protein [BBa_K4165003] that bind to Docs [BBa_K3396000] to form the protac system with linker GSGS [BBa_K4165065]
 
Fusion protein consists of Tau binding peptide (M4W39) for tau targeting and Coh2 protein [BBa_K4165003] that bind to Docs [BBa_K3396000] to form the protac system with linker GSGS [BBa_K4165065]
  
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===Usage and Biology===
 
===Usage and Biology===
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Protac system is known to be a promising one for targeting and signalling many diseases and biological application so according to our project aim. we introduced this fusion protein with different linker length and tau binding peptide to be a target for degradation of Tau aggregations. Furthermore, that can be validated by wet-lab results by in vitro or in vivo testing.
  
 
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<span class='h3bb'>Sequence and Features</span>
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===<span class='h3bb'>Sequence and Features</span>===
 
<partinfo>BBa_K4165208 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4165208 SequenceAndFeatures</partinfo>
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===3D Modeling and Ranking===
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This fused protein was modeled using Alphafold2.0 software and after running our quality assessment code it got a score of 4 out of 6 according to the parameters of the QA code: C-beta deviations, clash score, Molprobity, Ramachandran favored, Ramachandran outliers, Q mean 4, and Q mean 6.
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This protein was excluded due to its low QA score as compared to other models.
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<p><img src="https://static.igem.wiki/teams/4165/wiki/parts-registry/g2s.png" style="margin-left:200px;" alt="" width="500" /></p>
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                        Figure 1. Predicted 3D structure of CoH2-(GSGS)-M4W39 protein Visualized by Pymol.
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Latest revision as of 08:54, 13 October 2022


Coh2 linked with tau binding peptide (M4W39) and GSGS linker

Fusion protein consists of Tau binding peptide (M4W39) for tau targeting and Coh2 protein [BBa_K4165003] that bind to Docs [BBa_K3396000] to form the protac system with linker GSGS [BBa_K4165065]

Usage and Biology

Protac system is known to be a promising one for targeting and signalling many diseases and biological application so according to our project aim. we introduced this fusion protein with different linker length and tau binding peptide to be a target for degradation of Tau aggregations. Furthermore, that can be validated by wet-lab results by in vitro or in vivo testing.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


3D Modeling and Ranking

This fused protein was modeled using Alphafold2.0 software and after running our quality assessment code it got a score of 4 out of 6 according to the parameters of the QA code: C-beta deviations, clash score, Molprobity, Ramachandran favored, Ramachandran outliers, Q mean 4, and Q mean 6. This protein was excluded due to its low QA score as compared to other models. 

                        Figure 1. Predicted 3D structure of CoH2-(GSGS)-M4W39 protein Visualized by Pymol.