Difference between revisions of "Part:BBa K4307027"

 
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<partinfo>BBa_K4307027 short</partinfo>
 
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This part is the promoter regulated by PmrB/A two-component system in <i>E.coli</i>. When PmrA (BBa_K4307026) is phosphorylated, it will combine with pmrC and start the gene expression. In Tsinghua 2022 project, it was used in affipmrABC (BBa_K4307028） system.
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<partinfo>BBa_K4307027 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4307027 SequenceAndFeatures</partinfo>
  
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===Functional Parameters===
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<partinfo>BBa_K4307000 parameters</partinfo>
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<h2>Characterization </h2>
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<p>The following figure demonstrates our successful construction.</p>
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<br>
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https://static.igem.wiki/teams/4307/wiki/wiki-parts-files/mainpage/bba-k4307027-gel.png<br>
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                <b>Figure 1: </b> <b>The construction results of PmrC.</b>
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
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Revision as of 15:54, 12 October 2022


PmrC

This part is the promoter regulated by PmrB/A two-component system in E.coli. When PmrA (BBa_K4307026) is phosphorylated, it will combine with pmrC and start the gene expression. In Tsinghua 2022 project, it was used in affipmrABC (BBa_K4307028） system.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 372
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Characterization

The following figure demonstrates our successful construction.


bba-k4307027-gel.png

               Figure 1:  The construction results of PmrC.