Difference between revisions of "Part:BBa K4158013:Experience"
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how you used this part and how it worked out. | how you used this part and how it worked out. | ||
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| + | <i>in vitro</i> experiments were conducted. tube3 shows fluorescence before incubation without ITPG. On the other hand, in tube3-1 at 150 min after the addition of IPTG, the activity of gfp was decreased. Therefore, it is thought that the activity of gfp is regulated by the addition of IPTG. When IPTG is added, EdcR is purified by PET-T7-edcR. This result suggests that the suppression of gfp is caused by the generation of EdcR. | ||
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| + | [[File:Waseda Tokyo FITC values before incubation (left) and after 150min when FITC is 5M.png|500px|thumb|center|Fig. 1. FITC values before incubation (left) and after 150min when FITC is 5M]] | ||
===User Reviews=== | ===User Reviews=== | ||
Latest revision as of 15:51, 12 October 2022
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how you used this part and how it worked out.
in vitro experiments were conducted. tube3 shows fluorescence before incubation without ITPG. On the other hand, in tube3-1 at 150 min after the addition of IPTG, the activity of gfp was decreased. Therefore, it is thought that the activity of gfp is regulated by the addition of IPTG. When IPTG is added, EdcR is purified by PET-T7-edcR. This result suggests that the suppression of gfp is caused by the generation of EdcR.
_and_after_150min_when_FITC_is_5M.png)
User Reviews
UNIQ983b589ffb4d221c-partinfo-00000000-QINU UNIQ983b589ffb4d221c-partinfo-00000001-QINU