Difference between revisions of "Part:BBa K4307012"

 
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Originated from <i>Lactococcus lactis subsp. Lactis</i>, NisC is involved in the post-translational modification of the lantibiotic nisin. We managed to express NisC in <i>E.coli</i> for post-translational modification of NisA-affi fusion protein.<br>
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Functions together with NisA-affi(BBa_K4307010) and NisB(BBa_K4307011), it forms composite part NisA-affi-NisB+NisC (BBa_K4307014) to produce functional NisA-affibody peptide.
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<partinfo>BBa_K4307012 SequenceAndFeatures</partinfo>
 
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===Functional Parameters===
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<partinfo>BBa_K4307012 parameters</partinfo>
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<h2>Characterization</h2>
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<p>The following figure demonstrates our successful construction.</p>
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<br>
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https://static.igem.wiki/teams/4307/wiki/wiki-parts-files/mainpage/bba-k4307012-gel.png<br>
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                <b>Figure 1: </b> <b>The construction results of NisC.</b>
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Revision as of 15:47, 12 October 2022


NisC

Originated from Lactococcus lactis subsp. Lactis, NisC is involved in the post-translational modification of the lantibiotic nisin. We managed to express NisC in E.coli for post-translational modification of NisA-affi fusion protein.
Functions together with NisA-affi(BBa_K4307010) and NisB(BBa_K4307011), it forms composite part NisA-affi-NisB+NisC (BBa_K4307014) to produce functional NisA-affibody peptide.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 541
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 541
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 541
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 541
  • 1000
    COMPATIBLE WITH RFC[1000]


Characterization

The following figure demonstrates our successful construction.


bba-k4307012-gel.png

               Figure 1:  The construction results of NisC.