Difference between revisions of "Part:BBa K4158013:Experience"
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===Applications of BBa_K4158013=== | ===Applications of BBa_K4158013=== | ||
| + | tube3 shows fluorescence before incubation without ITPG. On the other hand, in tube3-1 at 150 min after the addition of IPTG, the activity of gfp was decreased. Therefore, it is thought that the activity of gfp is regulated by the addition of IPTG. When IPTG is added, EdcR is purified by PET-T7-edcR. This result suggests that the suppression of gfp is caused by the generation of EdcR. | ||
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| + | [File:Waseda Tokyo FITC values before incubation (left) and after 150min when FITC is 5M.png|500px|thumb|center|Fig. 1. FITC values before incubation (left) and after 150min when FITC is 5M] | ||
===User Reviews=== | ===User Reviews=== | ||
Revision as of 15:45, 12 October 2022
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Applications of BBa_K4158013
tube3 shows fluorescence before incubation without ITPG. On the other hand, in tube3-1 at 150 min after the addition of IPTG, the activity of gfp was decreased. Therefore, it is thought that the activity of gfp is regulated by the addition of IPTG. When IPTG is added, EdcR is purified by PET-T7-edcR. This result suggests that the suppression of gfp is caused by the generation of EdcR.
[File:Waseda Tokyo FITC values before incubation (left) and after 150min when FITC is 5M.png|500px|thumb|center|Fig. 1. FITC values before incubation (left) and after 150min when FITC is 5M]
User Reviews
UNIQ90be63f236e68310-partinfo-00000000-QINU UNIQ90be63f236e68310-partinfo-00000001-QINU