Difference between revisions of "Part:BBa K4361111"
 
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<partinfo>BBa_K4361111 short</partinfo>
 
<partinfo>BBa_K4361111 short</partinfo>
  
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The <i> blc </i> operator sequence, comparable to [[Part:BBa_K4361100]] but lacking some nucleotides at the 5' and 3' site. This sequence is made for the insertion into a plasmid to act as a repressor for the expression of downstream genes.
  
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Similar to [[Part:BBa_K4361001]] (lacking the first four nucleotides on both the 5' and 3' ends), but instead of being used as a separate oligo, this sequence is meant to be incorporated into a genetic circuit to let BlcR ([[Part:BBa_K4361100]]) act as a repressor for expression of downstream genes. See also [[Part:BBa_K4361115]].
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In [[Part:BBa_K4361115]] this part is used to create an expression vetor with the super folded GFP gene upfront the <i> blc </i> operator.
  
 
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===Usage and Biology===
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<span class='h3bb'><h3>Sequence and Features</h3></span>
 
<span class='h3bb'><h3>Sequence and Features</h3></span>
 
<partinfo>BBa_K4361111 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4361111 SequenceAndFeatures</partinfo>
  
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<h3> Usage in Biology </h3>
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BlcR is an allosteric transcription factor BlcR from the bacterium Agrobacterium tumefaciens. This plant bacterium is able to use GBL, a precursor of GHB, as an energy source. In the absence of GBL, GHB or SSA, BlcR will bind to the blc operator and acts as a repressor for the transcription of the blc proteins. When GBL, GHB or SSA binds to BlcR, it is released from the DNA and the blcA, blcB and blcC proteins are transcribed and digest GBL to succinate ( Figure 1 ).
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<a href="https://static.igem.wiki/teams/4361/wiki/design/blcr-mechanism.png"><img src="https://static.igem.wiki/teams/4361/wiki/design/blcr-mechanism.png" style="width:500px;margin-left:175px"></a>
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<figcaption> <b>Figure 1.</b> The regulatory mechanism of BlcR on the <i>blc</i> operon and the pathway from gamma-butyrolactone to succinic acid of <i>Agrobacterium tumefaciens</i>.</figcaption>
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Latest revision as of 15:42, 12 October 2022


Blc operator sequence

The blc operator sequence, comparable to Part:BBa_K4361100 but lacking some nucleotides at the 5' and 3' site. This sequence is made for the insertion into a plasmid to act as a repressor for the expression of downstream genes.

In Part:BBa_K4361115 this part is used to create an expression vetor with the super folded GFP gene upfront the blc operator.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Usage in Biology

BlcR is an allosteric transcription factor BlcR from the bacterium Agrobacterium tumefaciens. This plant bacterium is able to use GBL, a precursor of GHB, as an energy source. In the absence of GBL, GHB or SSA, BlcR will bind to the blc operator and acts as a repressor for the transcription of the blc proteins. When GBL, GHB or SSA binds to BlcR, it is released from the DNA and the blcA, blcB and blcC proteins are transcribed and digest GBL to succinate ( Figure 1 ).

Figure 1. The regulatory mechanism of BlcR on the blc operon and the pathway from gamma-butyrolactone to succinic acid of Agrobacterium tumefaciens.