Difference between revisions of "Part:BBa K3317006"

 
 
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<partinfo>BBa_K3317006 short</partinfo>
 
<partinfo>BBa_K3317006 short</partinfo>
  
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Sensitive promoter against vanillinic acid
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Sensitive promoter against vanillinic acid and presents its maximum induction in the presence of 100mM RPU 3 of vanillinic acid with a leak of 2.4x10-3 RPU in absence of induction (Meyer, A. J <i> et. al</i> 2019) this part needs its regulatory protein (<partinfo>K3317004 </partinfo>)
  
 
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<partinfo>BBa_K3317006 parameters</partinfo>
 
<partinfo>BBa_K3317006 parameters</partinfo>
 
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===2022 BNSC-China improvement===
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We tested the response of the food additive vanillic acid response promoter PvanCC and found that its activation ploidy was low compared to the original literature, and to further increase its activation ploidy, we used the literature-reported, RNA-coordinated THS strategy [BBa_K4237022] to increase the activation ploidy from approximately 20-fold to 34-fold.

Latest revision as of 15:24, 12 October 2022


Inducible pVanCC promoter

Sensitive promoter against vanillinic acid and presents its maximum induction in the presence of 100mM RPU 3 of vanillinic acid with a leak of 2.4x10-3 RPU in absence of induction (Meyer, A. J et. al 2019) this part needs its regulatory protein (BBa_K3317004)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 18
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 4
    Illegal BamHI site found at 42
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


2022 BNSC-China improvement

We tested the response of the food additive vanillic acid response promoter PvanCC and found that its activation ploidy was low compared to the original literature, and to further increase its activation ploidy, we used the literature-reported, RNA-coordinated THS strategy [BBa_K4237022] to increase the activation ploidy from approximately 20-fold to 34-fold.