Difference between revisions of "Part:BBa K4195179"

(Usage and Design)
 
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====Reference====
 
====Reference====
 
1. J. M. S. Lazarte ''et al.'', Passive Immunization with Recombinant Antibody VLRB-PirA(vp)/PirB(vp)-Enriched Feeds against ''Vibrio parahaemolyticus'' Infection in ''Litopenaeus vannamei'' Shrimp. ''Vaccines (Basel)''. '''9''',  (2021).
 
1. J. M. S. Lazarte ''et al.'', Passive Immunization with Recombinant Antibody VLRB-PirA(vp)/PirB(vp)-Enriched Feeds against ''Vibrio parahaemolyticus'' Infection in ''Litopenaeus vannamei'' Shrimp. ''Vaccines (Basel)''. '''9''',  (2021).
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K4195179 SequenceAndFeatures</partinfo>

Latest revision as of 15:17, 12 October 2022

Biology

This sequence is a conserved region of toxin gene pirA(1). It’s used as the detection target of RENDR system.
Ribozyme ENabled Detection of RNA (RENDR)
RENDR is a high-performing, plug-and-play RNA-sensing platform. RENDR utilizes a split variant of the Tetrahymena thermophila ribozyme by synthetically splitting it into two non-functional fragments. Two fragments are each appended with designed RNA guide sequences, which can interact with the RNA input of interest. The split ribozyme is then inserted within a desired gene output. When binded with the RNA input, two transcribed split ribozyme fragments are triggered to self-splice and thus the intact transcript of the protein output will form.
T--XMU-China--RENDR.png
Fig. 1 Schematic illustration of RENDR.

Usage and Design

This part is used as the target of the RENDR detection system. For toxin pirA, we designed BBa_K4195141, BBa_K4195142, BBa_K4195145, BBa_K4195146, BBa_K4195156, BBa_K4195157, BBa_K4195160, BBa_K4195161, BBa_K4195168, BBa_K4195169, BBa_K4195172, BBa_K4195173. Other related parts are as followings: BBa_K4195151, BBa_K4195183, BBa_K4195184, BBa_K4195185, BBa_K4195186.

Reference

1. J. M. S. Lazarte et al., Passive Immunization with Recombinant Antibody VLRB-PirA(vp)/PirB(vp)-Enriched Feeds against Vibrio parahaemolyticus Infection in Litopenaeus vannamei Shrimp. Vaccines (Basel). 9, (2021).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 205
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]