Difference between revisions of "Part:BBa K4239001"
 
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<partinfo>BBa_K4239001 short</partinfo>
 
<partinfo>BBa_K4239001 short</partinfo>
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<h2>Sequence and Features</h2>
 
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<partinfo>BBa_K4239001 SequenceAndFeatures</partinfo>
 
  
 
<html>
 
<html>
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<br>
  
 
<h2>Description</h2>  
 
<h2>Description</h2>  
  
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<p><i>fiatluxC</i> is made to be used with <i>fiatluxD</i> and <i>fiatluxE</i>. It codes for a subpart fatty acid reductase. With the subparts coding from <i>fiatluxD</i> and <i>fiatluxE</i>, they form a complex that recycles fatty acids to fatty aldehydes. Fatty aldehydes will be used as a substrat for the luciferase protein.</p>
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<p><i>fiatluxC</i> is to be used with  
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<i>fiatluxD</i> <a href="https://parts.igem.org/Part:BBa_K4239002" class="pr-0" target="_blank">(BBa_K4239002)</a>
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and <i>fiatluxE</i> <a href="https://parts.igem.org/Part:BBa_K4239005" class="pr-0" target="_blank">(BBa_K4239005)</a>.  
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It codes for a subpart fatty acid reductase. With the subparts encoded by <i>fiatluxD</i> and <i>fiatluxE</i>, they form a complex that recycles fatty acids to fatty aldehydes. Fatty aldehydes will be used as a substrate for the luciferase protein.</p>
  
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<p>The systeme <i>fiatluxC/fiatluxD/fiatluxE</i> is made to be used with <i>fiatluxA</i> and <i>fiatluxB</i>, gathered in the <i>fiatluxCDABE</i> operon.</p>
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<p>The system <i>fiatluxC/fiatluxD/fiatluxE</i> is to be used with  
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<i>fiatluxA</i> <a href="https://parts.igem.org/Part:BBa_K4239003" class="pr-0" target="_blank">(BBa_K4239003)</a>
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and <i>fiatluxB</i> <a href="https://parts.igem.org/Part:BBa_K4239004" class="pr-0" target="_blank">(BBa_K4239004)</a>,
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gathered in the <i>fiatluxCDABE</i> <a href="https://parts.igem.org/Part:BBa_K4239008" class="pr-0" target="_blank">(BBa_K4239008)</a>
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operon.</p>
  
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<p><i>Fiatlux</i> genes come from <i>ilux</i> genes (C, D, A, B, E). They were modified to remove every Igem restriction site (EcoR1, Xba1, Spe1 and Pst1) included in genes. They were also adapted to include the biobrick format.</p>
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<p><i>fiatlux</i> genes come from <i>ilux</i> genes (C, D, A, B, E). They were modified to remove every iGEM restriction site (EcoRI, XbaI, SpeI and PstI) included in genes. They were also adapted to include the biobrick format.</p>
  
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<p>The <i>ilux</i> operon was born from a mutated natural luminescence operon present in the bacteria P.luminescens: the <i>lux</i> operon. These mutations were error-prone PCR induced according to Gregor et al.’s study in 2018 (Gregor et al. 2018). The aim was to create a system of genes that produced more light.</p>
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<p>The <i>ilux</i> operon was born from a mutated natural luminescence operon present in the bacteria <i>P.luminescens</i>: the <i>lux</i> operon. These mutations were error-prone PCR induced according to Gregor et al.’s study in 2018 (Gregor et al. 2018). The aim was to create a system of genes that produced more light than the <i>lux</i> system.</p>
  
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<br>
  
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<h2>Construction</h2>  
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</html>
  
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<p><i>fiatluxC</i> was directly synthesized with <i>fiatluxD</i> and the promoter J23117 <a href="https://parts.igem.org/Part:BBa_J23117" class="pr-0" target="_blank">(BBa_J23117)</a>. The description of the synthesis is on the page part <a href="https://parts.igem.org/Part:BBa_K4239006" class="pr-0" target="_blank">(BBa_K4239006)</a>.</p>
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<h2>Sequence and Features</h2>
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<partinfo>BBa_K4239001 SequenceAndFeatures</partinfo>
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<html>
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<br>
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<h2>Construction</h2>  
  
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<p><i>fiatluxC</i> was directly synthesized with <i>fiatluxD</i> and the promoter J23117 <a href="https://parts.igem.org/Part:BBa_J23117" class="pr-0" target="_blank">(BBa_J23117)</a>. The description of the synthesis is on the following page part <i>fiatluxCD</i> <a href="https://parts.igem.org/Part:BBa_K4239006" class="pr-0" target="_blank">(BBa_K4239006)</a>.</p>
  
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METTRE LES PRIMERS UTILISés
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<br>
  
 
<h2>References</h2>  
 
<h2>References</h2>  

Latest revision as of 14:56, 12 October 2022


Enhanced luciferase subunits fiatluxC


Description

fiatluxC is to be used with fiatluxD (BBa_K4239002) and fiatluxE (BBa_K4239005). It codes for a subpart fatty acid reductase. With the subparts encoded by fiatluxD and fiatluxE, they form a complex that recycles fatty acids to fatty aldehydes. Fatty aldehydes will be used as a substrate for the luciferase protein.

The system fiatluxC/fiatluxD/fiatluxE is to be used with fiatluxA (BBa_K4239003) and fiatluxB (BBa_K4239004), gathered in the fiatluxCDABE (BBa_K4239008) operon.

fiatlux genes come from ilux genes (C, D, A, B, E). They were modified to remove every iGEM restriction site (EcoRI, XbaI, SpeI and PstI) included in genes. They were also adapted to include the biobrick format.

The ilux operon was born from a mutated natural luminescence operon present in the bacteria P.luminescens: the lux operon. These mutations were error-prone PCR induced according to Gregor et al.’s study in 2018 (Gregor et al. 2018). The aim was to create a system of genes that produced more light than the lux system.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1311


Construction

fiatluxC was directly synthesized with fiatluxD and the promoter J23117 (BBa_J23117). The description of the synthesis is on the following page part fiatluxCD (BBa_K4239006).


References

Gregor C, Gwosch KC, Sahl SJ, Hell SW. Strongly enhanced bacterial bioluminescence with the ilux operon for single-cell imaging. Proc Natl Acad Sci U S A. 2018 Jan 30;115(5):962-967. doi: 10.1073/pnas.1715946115. Epub 2018 Jan 16. PMID: 29339494; PMCID: PMC5798359.