Difference between revisions of "Part:BBa J364009"
(→‎Improvement by 2022 team HUS_United)
Line 13: Line 13:
  
 
==Improvement by 2022 team HUS_United==
 
==Improvement by 2022 team HUS_United==
 +
 +
This year our team wishes to introduce a new kind of basic parts-degradation tuning RNAs to the iGEM community. The dtRNAs are reported to be capable of modulating mRNA degradation rates by resisting or facilitating endogenous RNase digestion. We plan to add this compact(10-60 bp) component upstream of the RBS B0032 in this part, in order to enhance GFP reporter signals without introducing metabolic burden to the host organism.
 +
  
  

Revision as of 14:46, 12 October 2022


Test Device 6 for 2018 InterLab Study

Test Device 6 expresses GFP under the control of a constitutive promoter from the Anderson collection.


Interlab 2022 Experiment 3 Device

  • Test Device 6 for Experiment 3 of the 2022 iGEM Interlab

The following device is used in the iGEM 2022 InterLaboratory Study for Experiment 3.


Improvement by 2022 team HUS_United

This year our team wishes to introduce a new kind of basic parts-degradation tuning RNAs to the iGEM community. The dtRNAs are reported to be capable of modulating mRNA degradation rates by resisting or facilitating endogenous RNase digestion. We plan to add this compact(10-60 bp) component upstream of the RBS B0032 in this part, in order to enhance GFP reporter signals without introducing metabolic burden to the host organism.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 705