Difference between revisions of "Part:BBa K4344050"

 
 
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<partinfo>BBa_K4344050 short</partinfo>
 
<partinfo>BBa_K4344050 short</partinfo>
  
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Plasmid with the same sequence as part BBa_K4344046, except that Tac-LacO-SD (BBa_K4344082) is added to enhance the expression of p19. For further information see our wiki https://2022.igem.wiki/heidelberg/experiments.
  
 
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Latest revision as of 14:45, 12 October 2022


pUC19-p19-6xHis-siRNA empty (Tac-LacO-SD)

Plasmid with the same sequence as part BBa_K4344046, except that Tac-LacO-SD (BBa_K4344082) is added to enhance the expression of p19. For further information see our wiki https://2022.igem.wiki/heidelberg/experiments.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 551
    Illegal XbaI site found at 1032
    Illegal XbaI site found at 1099
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 551
    Illegal NotI site found at 1140
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 551
    Illegal BamHI site found at 1233
    Illegal XhoI site found at 1093
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 551
    Illegal XbaI site found at 1032
    Illegal XbaI site found at 1099
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 551
    Illegal XbaI site found at 1032
    Illegal XbaI site found at 1099
  • 1000
    COMPATIBLE WITH RFC[1000]