Difference between revisions of "Part:BBa K4361116"
 
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===Usage and Biology===
 
===Usage and Biology===
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<h3> Usage and Biology </h3>
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<partinfo>BBa_K4361116 parameters</partinfo>
 
<partinfo>BBa_K4361116 parameters</partinfo>
 
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<h3> References </h3>
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[1] Pan, Y., Fiscus, V., Meng, W., Zheng, Z., Zhang, L.-H., Fuqua, C. and Chen, L. (2011). The Agrobacterium tumefaciens Transcription Factor BlcR Is Regulated via Oligomerization. The Journal of Biological Chemistry, [online] 286(23), pp.20431–20440. doi:10.1074/jbc.M110.196154

Latest revision as of 14:40, 12 October 2022


GHB / GBL biosensor reporter, deletion in Blc operator

This part shows the GHB / GBL reporter system designed by the Bielefeld-CeBiTec iGEM 2015 team (Part:BBa_K1758376) but with a deletion in the blc operator sequence. Deletion is inserted with primers Part:BBa_K4361112 and Part:BBa_K4361113.

It is known from literature that this mutation disrupts the binding between BlcR and the blc operator sequence [1]. This way BlcR cannot inhibit downstream gene expression.

The mutated blc operator sequence is incorperated in a superfolded GFP production plasmid, seePart:BBa_K4361115.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 121


References

[1] Pan, Y., Fiscus, V., Meng, W., Zheng, Z., Zhang, L.-H., Fuqua, C. and Chen, L. (2011). The Agrobacterium tumefaciens Transcription Factor BlcR Is Regulated via Oligomerization. The Journal of Biological Chemistry, [online] 286(23), pp.20431–20440. doi:10.1074/jbc.M110.196154