Difference between revisions of "Part:BBa K4390026"

 
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<partinfo>BBa_K4390026 short</partinfo>
 
<partinfo>BBa_K4390026 short</partinfo>
  
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This composite part is used for silica immobilisation of our metallothionein.  
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'''This part is not compatible with BioBrick RFC10 assembly but is compatible with the iGEM Type IIS Part standard [[Help:Standards/Assembly/Type_IIS|which is also accepted by iGEM.]]'''
  
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<!-- Add more about the biology of this part here
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==Usage and Biology==
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===Usage and Biology===
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SB7 is a 7-amino-acid silica-binding motif from the spore coat protein CotB1 of Bacillus cereus. According to studies, its three arginine residues are important for binding to silica surfaces. We tagged this sequence to the N-terminus of the metallothionein of Mytilus edulis. Therefore, when the lysate from a cell culture expressing SB7-tagged metallothionein is mixed with silica beads followed by washing off the supernatant, we would be left with metallothioneins bound to the silica beads. This entire construct could then be applied as an ultrafiltration device for removing heavy metals from the wastewater. This is one of the three types of silica-binding tags we explored for immobilising proteins to silica beads.
  
 
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<span class='h3bb'>Sequence and Features</span>
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==<span class='h3bb'>Sequence and Features</span>==
 
<partinfo>BBa_K4390026 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4390026 SequenceAndFeatures</partinfo>
  

Revision as of 14:35, 12 October 2022


SB7-tagged M. edulis Metallothionein

This part is not compatible with BioBrick RFC10 assembly but is compatible with the iGEM Type IIS Part standard which is also accepted by iGEM.

Usage and Biology

SB7 is a 7-amino-acid silica-binding motif from the spore coat protein CotB1 of Bacillus cereus. According to studies, its three arginine residues are important for binding to silica surfaces. We tagged this sequence to the N-terminus of the metallothionein of Mytilus edulis. Therefore, when the lysate from a cell culture expressing SB7-tagged metallothionein is mixed with silica beads followed by washing off the supernatant, we would be left with metallothioneins bound to the silica beads. This entire construct could then be applied as an ultrafiltration device for removing heavy metals from the wastewater. This is one of the three types of silica-binding tags we explored for immobilising proteins to silica beads.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 11
    Illegal NheI site found at 34
    Illegal NotI site found at 73
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]