Difference between revisions of "Part:BBa K4344043"

 
 
Line 3: Line 3:
 
<partinfo>BBa_K4344043 short</partinfo>
 
<partinfo>BBa_K4344043 short</partinfo>
  
−
Placeholder
+
Plasmid backbone of pRK5. Used for expression of UL19 in HeLa cells.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
Line 11: Line 11:
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K4344043 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4344043 SequenceAndFeatures</partinfo>
 +
 +
The pRK5 has not one single version, but different versions of the plasmid have been published and are available to purchase. After cross referencing with the supplier Addgene, we noticed that there are multiple proposed plasmid vector maps (see notes at Addgene plasmid 3944). For further information look in our Wiki.
  
  

Latest revision as of 14:25, 12 October 2022


pRK5

Plasmid backbone of pRK5. Used for expression of UL19 in HeLa cells.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 919
    Illegal XbaI site found at 936
    Illegal SpeI site found at 28
    Illegal PstI site found at 948
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 919
    Illegal SpeI site found at 28
    Illegal PstI site found at 948
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 919
    Illegal BamHI site found at 930
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 919
    Illegal XbaI site found at 936
    Illegal SpeI site found at 28
    Illegal PstI site found at 948
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 919
    Illegal XbaI site found at 936
    Illegal SpeI site found at 28
    Illegal PstI site found at 948
    Illegal NgoMIV site found at 1908
  • 1000
    COMPATIBLE WITH RFC[1000]

The pRK5 has not one single version, but different versions of the plasmid have been published and are available to purchase. After cross referencing with the supplier Addgene, we noticed that there are multiple proposed plasmid vector maps (see notes at Addgene plasmid 3944). For further information look in our Wiki.