Difference between revisions of "Part:BBa K3684002"

Revision as of 14:23, 12 October 2022

Rat Diamine Oxidase

Diamine Oxidases, DAO, are widespread among living organisms and found in both microorganisms and in plants and animals. DAO is responsible for histamine metabolism: it was also named histaminase because it was identified as the enzyme that cleared histamine from minced lung and liver samples. DAO was proposed to represent a safeguard against deleterious effects from exogenous amines. For example, it is related to histamine detoxification in the intestinal tract and to putrescine and histamine detoxification in placental tissues. And rat Diamine Oxidase, rDAO, plays an important role in oxidizing histamine in rat. This protein can be expressed and purified to degrade histamine.

Contribution: HKUST 2022

Team HKUST 2022 add a N-terminal pelB leader sequence in rDAO. Adding pelB tag to rDAO increases the functional expression of rDAO in our DH5α chassis. To know more about our experiment result, refer to composite parts BBa_K4225022 and BBa_K4225007, while to know more about effect on pelB on rDAO in our DH5α chassis, refer to our wiki page.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1004
Illegal BamHI site found at 1932
Illegal BamHI site found at 2186
Illegal XhoI site found at 802
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 ==Contribution: HKUST 2022==
-Team HKUST 2022 add a N-terminal pelB leader sequence in rDAO. Adding pelB tag to rDAO increases the functional expression of rDAO in our DH5α chassis. To know more about our experiment result, refer to composite parts [https://parts.igem.org/Part:BBa_K4225022 BBa_K4225022] [https://parts.igem.org/Part:BBa_K4225007 BBa_K4225007], while to know more about effect on pelB on rDAO in our DH5α chassis, refer to our [https://2022.igem.wiki/hkust/design wiki page.]
+Team HKUST 2022 add a N-terminal pelB leader sequence in rDAO. Adding pelB tag to rDAO increases the functional expression of rDAO in our DH5α chassis. To know more about our experiment result, refer to composite parts [https://parts.igem.org/Part:BBa_K4225022 BBa_K4225022] and [https://parts.igem.org/Part:BBa_K4225007 BBa_K4225007], while to know more about effect on pelB on rDAO in our DH5α chassis, refer to our [https://2022.igem.wiki/hkust/design wiki page.]
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