Difference between revisions of "Part:BBa K4160017"
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| − | <h2> | + | <h2>Usage & biology</h2> |
| − | <a href="https://2022.igem.wiki/tu-eindhoven/">TU-Eindhoven 2022</a> used this composite part as an output of the Generalized Extracellular Molecule Sensor (GEMS) system that is developed by Scheller et al., 2018.<sup>1</sup> Upon activation of the GEMS receptor, STAT-induced IL-10 will be expressed and secreted via the JAK/STAT signaling pathway. In the project of TU-Eindhoven 2022, IL-10 was used to achieve the expression of a therapeutically relevant agent upon activation of the GEMS receptor (<a href="https://parts.igem.org/Part:BBa_K4160008">BBa_K4160008</a>). STAT-induced IL-10 was expressed in a pLS13 plasmid.</p><br> | + | <a href="https://2022.igem.wiki/tu-eindhoven/">TU-Eindhoven 2022</a> used this composite part as an output of the Generalized Extracellular Molecule Sensor (GEMS) system that is developed by Scheller et al., 2018.<sup>1</sup> Upon activation of the GEMS receptor, STAT-induced IL-10 will be expressed and secreted via the JAK/STAT signaling pathway. In the project of <a href="https://2022.igem.wiki/tu-eindhoven/">TU-Eindhoven 2022,</a> IL-10 was used to achieve the expression of a therapeutically relevant agent upon activation of the GEMS receptor (<a href="https://parts.igem.org/Part:BBa_K4160008">BBa_K4160008</a>). STAT-induced IL-10 was expressed in a pLS13 plasmid.</p><br> |
<h2>Characterization </h2> | <h2>Characterization </h2> | ||
Latest revision as of 14:19, 12 October 2022
STAT-induced IL-10
This composite part encodes for STAT-induced IL-10 and is composed of the basic parts STAT promotor (BBa_K4160006) and IL-10 (BBa_K4160007).
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 209
Illegal XbaI site found at 233
Illegal PstI site found at 53 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 209
Illegal PstI site found at 53 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 209
Illegal BamHI site found at 227 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 209
Illegal XbaI site found at 233
Illegal PstI site found at 53 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 209
Illegal XbaI site found at 233
Illegal PstI site found at 53 - 1000COMPATIBLE WITH RFC[1000]
Usage & biology
TU-Eindhoven 2022 used this composite part as an output of the Generalized Extracellular Molecule Sensor (GEMS) system that is developed by Scheller et al., 2018.1 Upon activation of the GEMS receptor, STAT-induced IL-10 will be expressed and secreted via the JAK/STAT signaling pathway. In the project of TU-Eindhoven 2022, IL-10 was used to achieve the expression of a therapeutically relevant agent upon activation of the GEMS receptor (BBa_K4160008). STAT-induced IL-10 was expressed in a pLS13 plasmid.Characterization
Characterization of STAT-induced IL-10 expression by the GEMS system can be found on the BBa_K4160008 page.
References