Difference between revisions of "Part:BBa K4488018"

Latest revision as of 14:15, 12 October 2022

fuBFP

freeuse Blue Fluorescent Protein, a blue fluorescent protein that fluoresces under long-wave UV.

This part is a variant of fuGFP (Part:BBa K3814004) which was originally designed to create a GFP variant not protected by patents and able to facilitate any kind of research. Using PCR with degenerate primers we introduced the Y67H mutation in the fluorophore to create fuBFP. The variant has an excitation (380 nm) and emission peak (450 nm) at a shorter wavelength (figure 1). We have also created a fusion protein of fuBFP connected to a cellulose-binding domain which allows it to be immobilised on cellulose (Part:BBa K4488021). There is a variant of fuBFP made from fuGFP with S66T instead which has similar spectral profiles but is less fluorescent by eye under UV.

Usage and Biology

Figure 1. Absorption and emission spectra for fuBFP and its threonine variant. Fluorescence data was measured in a plate reader for proteins obtained from E. coli colonies.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 151
1000
COMPATIBLE WITH RFC[1000]

@@ Line 5: / Line 5: @@
 freeuse Blue Fluorescent Protein, a blue fluorescent protein that fluoresces under long-wave UV.
-This part is a variant of fuGFP (Part:BBa K3814004) which was originally designed to create a GFP variant not protected by patents and able to facilitate any kind of research. Using PCR with degenerate primers we introduced the Y67H mutation in the fluorophore to create fuBFP. The variant has an excitation (380 nm) and emission peak (450 nm) at a shorter wavelength (figure 1). We have also created a fusion protein of fuBFP connected to a cellulose-binding domain which allows it to be immobilised on cellulose (Part:BBa K4488021).
+This part is a variant of fuGFP (Part:BBa K3814004) which was originally designed to create a GFP variant not protected by patents and able to facilitate any kind of research. Using PCR with degenerate primers we introduced the Y67H mutation in the fluorophore to create fuBFP. The variant has an excitation (380 nm) and emission peak (450 nm) at a shorter wavelength (figure 1). We have also created a fusion protein of fuBFP connected to a cellulose-binding domain which allows it to be immobilised on cellulose (Part:BBa K4488021). There is a variant of fuBFP made from fuGFP with S66T instead which has similar spectral profiles but is less fluorescent by eye under UV.
 ===Usage and Biology===
-[[File:insert file name.png|centre|thumb|700px| Figure 1. Absorption and emission spectrum for fuBFP.]]
+[[File:fuBFP_spectra.jpg|centre|thumb|700px| Figure 1. Absorption and emission spectra for fuBFP and its threonine variant. Fluorescence data was measured in a plate reader for proteins obtained from E. coli colonies.]]