Difference between revisions of "Part:BBa K4317014"

Latest revision as of 14:01, 12 October 2022

PtrnQ

trnQ codes for arginine-specific tRNA from Basillus subtilis

PtrnQ was only twice as high as that from the P43 promoter.

Usage and Biology

We registered 5 promoters, 5 secretion signals, and 4 cellulose degrading enzymes as new basic parts. Our newly registered P2069M (BBa_K4317001) and PtrnQ (BBa_K4317004) constitutive promoters can be used in both Bacillus and E. coli. In particular, in Bacillus, these promoters showed higher transcriptional activity than the previously known P43 promoter.

Reference

Song, Yafeng, et al. "Promoter screening from Bacillus subtilis in various conditions hunting for synthetic biology and industrial applications." PloS one 11.7 (2016): e0158447.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 7
Illegal XbaI site found at 16
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 7
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 7
Illegal BglII site found at 13
Illegal BamHI site found at 1
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 7
Illegal XbaI site found at 16
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 7
Illegal XbaI site found at 16
1000
COMPATIBLE WITH RFC[1000]

@@ Line 16: / Line 16: @@
 https://static.igem.org/mediawiki/parts/thumb/5/51/Promoter_strength.png/800px-Promoter_strength.png
+We registered 5 promoters, 5 secretion signals, and 4 cellulose degrading enzymes as new basic parts. Our newly registered P2069M (BBa_K4317001) and PtrnQ (BBa_K4317004) constitutive promoters can be used in both Bacillus and E. coli. In particular, in Bacillus, these promoters showed higher transcriptional activity than the previously known P43 promoter.
 ==Reference==