Difference between revisions of "Part:BBa K4438901"
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crp_trigger_1_P1_phi29 (BBa_K4438901) is a single-stranded DNA having 23 nucleotides. Figure 1A) shows the secondary structure and minimum free energy. | crp_trigger_1_P1_phi29 (BBa_K4438901) is a single-stranded DNA having 23 nucleotides. Figure 1A) shows the secondary structure and minimum free energy. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
| + | The part crp_trigger_1_P1_phi29 (BBa_K4438901) has complete complementarity to the part Aptamer_crp (BBa_K4438900) and blocks the 3’ region of the CRP aptamer. Figure 1D) Shows the secondary structure of both parts hybridised at 37° Celsius. CRP binds with Aptamer_crp (BBa_K4438900) and displaces the crp_trigger_1_P1_phi29 (BBa_K4438901) [1]. This part has complete complementarity with part crp_Target_1(BBa_K4438902). Phi 29 DNA extension polymerase extends the template strand and in-vitro transcription of the duplex forms multiple broccoli light-up aptamers. | ||
| + | Different levels of CRP can be detected using all these three parts. | ||
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<partinfo>BBa_K4438901 parameters</partinfo> | <partinfo>BBa_K4438901 parameters</partinfo> | ||
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| + | ===References=== | ||
| + | Liu, Z., Luo, D., Ren, F., Ran, F., Chen, W., Zhang, B., ... & Chen, Q. (2019). Ultrasensitive fluorescent aptasensor for CRP detection based on the RNase H assisted DNA recycling signal amplification strategy. RSC advances, 9(21), 11960-11967. | ||
Revision as of 13:50, 12 October 2022
crp_Target_1
crp_trigger_1_P1_phi29 (BBa_K4438901) is a single-stranded DNA having 23 nucleotides. Figure 1A) shows the secondary structure and minimum free energy.
Usage and Biology
The part crp_trigger_1_P1_phi29 (BBa_K4438901) has complete complementarity to the part Aptamer_crp (BBa_K4438900) and blocks the 3’ region of the CRP aptamer. Figure 1D) Shows the secondary structure of both parts hybridised at 37° Celsius. CRP binds with Aptamer_crp (BBa_K4438900) and displaces the crp_trigger_1_P1_phi29 (BBa_K4438901) [1]. This part has complete complementarity with part crp_Target_1(BBa_K4438902). Phi 29 DNA extension polymerase extends the template strand and in-vitro transcription of the duplex forms multiple broccoli light-up aptamers. Different levels of CRP can be detected using all these three parts.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
Liu, Z., Luo, D., Ren, F., Ran, F., Chen, W., Zhang, B., ... & Chen, Q. (2019). Ultrasensitive fluorescent aptasensor for CRP detection based on the RNase H assisted DNA recycling signal amplification strategy. RSC advances, 9(21), 11960-11967.